Path: utzoo!utgpu!watserv1!watmath!att!news.cs.indiana.edu!bionet!UNIPAD.UNIPD.IT!BIOCOL From: BIOCOL@UNIPAD.UNIPD.IT Newsgroups: bionet.general Subject: Re: restriction enzyme sites in mammalian cell Message-ID: <9102230959.AA03533@genbank.bio.net> Date: 23 Feb 91 09:57:00 GMT Sender: daemon@genbank.bio.net Lines: 22 In BioNews Thomas Collet says: >I am trying to clone immunoglobulins from hybridoma cells and >am wondering whether I am using the best possible restriction >enzymes. Does anyone have a compilation of the cutting >frequencies of restriction enzymes in immunoglobulin genes (or >maybe just mammalian genes in general ?) Or maybe a literature >reference ? >Thanks I think you can try by yourself using stochastic computer methods (as I did), for example RND in BASIC. The frequence of cutting for each enzyme must be 1/4 exp n for each class with n bases of recogniction. I have no litterature but if you want I can send you, with hard mail, some table for 6 and 4 base-cut ters. Best Re: and Re:gards. Argenton Francesco Dip. di Biologia Universita' di Padova Via Trieste 75, 35100 Padova, ITALIA Brought to you by Super Global Mega Corp .com