Path: utzoo!utgpu!watserv1!watmath!att!news.cs.indiana.edu!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Human papillomavirus (HPV) type 16 genome, 5' virus-host integrated Message-ID: Date: 21 Feb 91 13:00:49 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 38 Approved: lear@genbank.bio.net Checksum: 25752 2 LOCUS PPHIJ1 200 bp ds-DNA VRL 21-FEB-1991 DEFINITION Human papillomavirus (HPV) type 16 genome, 5' virus-host integrated junction. ACCESSION D00486 KEYWORDS flanking sequences; integration; virus-host junction. SEGMENT 1 of 2 SOURCE Integrated human papillomavirus type 16 genome DNA in the cervical carcinoma cell line QG-U, clone UV411, BglII-SpeI fragment. ORGANISM Human papillomavirus Viridae; ds-DNA nonenveloped viruses; Papovaviridae; Papillomavirus. REFERENCE 1 (bases 1 to 200) AUTHORS Shirasawa,H., Tomita,Y., Fuse,A., Yamamoto,T., Tanzawa,H., Sekiya,S., Takamizawa,H. and Simizu,B. TITLE Structure and expression of an integrated human papillomavirus type 16 genome amplified in a cervical carcinoma cell line JOURNAL J. Gen. Virol. 70, 1913-1919 (1989) STANDARD full staff_entry COMMENT Sequence analyses revealed that the virus-host junctions reside at nucleotide positions 3741 and 4416. Part, 674 bp, of the viral genome (nucleotides 3742 to 4415) containing the putative early polyadenylation signal, was deleted. FEATURES Location/Qualifiers cellular <1..186 /note="QG-U cellular DNA" misc_feature 111..186 /note="AT-rich region" provirus 187..>200 /note="HPV DNA (containing L2 ORF region)" BASE COUNT 88 a 30 c 31 g 51 t ORIGIN Several bp downstream of BglII site. 1 catcagtacc atggaaccct actcagcaat aaaaagaaaa aactattgat aaatgcaaca 61 acttagatga acatcaagga aattatactg agtgaaagaa gccaatcccc aaaagataca 121 tatggcataa ttcaatttat tgaattatta ttattaattt catgaataaa attatagaga 181 tgaacagaac agggtcgggt //