Path: utzoo!utgpu!watserv1!watmath!uunet!spool.mu.edu!samsung!sdd.hp.com!usc!apple!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Non-A, non-B hepatitis virus (hepatitis C virus) genome, NS 5 Message-ID: Date: 23 Feb 91 13:03:45 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 56 Approved: lear@genbank.bio.net Checksum: 51643 3 LOCUS HPCJ2 235 bp ss-RNA VRL 23-FEB-1991 DEFINITION Non-A, non-B hepatitis virus (hepatitis C virus) genome, NS 5 region. ACCESSION D00587 KEYWORDS . SOURCE Hepatitis C virus (Japanese isolates HCV-J2a, -J2b), cDNA to viral RNA isolated from human specimen obtained by surgical resection of hepatocellular carcinomas. ORGANISM Hepatitis C virus Viridae; ss-RNA enveloped viruses; Positive strand RNA virus; Togaviridae incertae sedis. REFERENCE 1 (bases 1 to 235) AUTHORS Kato,N., Ohkoshi,S. and Shimotohno,K. TITLE Japanese isolates of the non-A, non-B hepatitis viral genome show sequence variations from the original isolate in the U.S.A JOURNAL Proc. Jpn. Acad. 65, 219-223 (1989) STANDARD full staff_entry COMMENT These data kindly submitted in computer readable form by: Nobuyuki Kato National Cancer Center Research Institute 5-1-1 Tsukiji, Chuo-ku Tokyo 104 Japan Phone: 03-542-2511 x4701 Fax: 813-545-3567 Japanese isolates (HCV-J1, -J2a and -J2b) showed 17 to 19% diversity of nucleotide sequence from the original isolate (HCV) in the United States. The nucleotide sequences of 7 clones derived from the Plasma (HCV-J1) were basically identical, but those of 8 clones derived from the liver specimen were classified into two types (HCV-J2a (5 clones) and J2b (3 clones)). FEATURES Location/Qualifiers virion <1..>235 /note="HCV-J2a,b genomic RNA" variation 46..46 /note="g in HCV-J2b; a in HCV-J2a" variation 79..79 /note="c in HCV-J2b; t in HCV-J2a" variation 83..83 /note="c in HCV-J2b; a in HCV-J2a" variation 88..88 /note="a in HCV-J2b; g in HCV-J2a" variation 94..94 /note="t in HCV-J2b; c in HCV-J2a" variation 97..97 /note="a in HCV-J2b; g in HCV-J2a" variation 233..233 /note="a in HCV-J2b; g in HCV-J2a" BASE COUNT 55 a 83 c 51 g 46 t ORIGIN 1 atcatgctcc tcaaacgtgt cggtcgcgca cgatgcatct ggcaagcggg tgtattacct 61 cacccgcgac cccaccaccc cccttgcacg agctgcatgg gagacagcca gacacactcc 121 agtcaactcc tggctaggca acatcatcat gtatgcgccc accttgtggg cgaggatgat 181 cctgatgacc cacttcttct ccatcctcct agcccaggag caacttgaaa aaacc //