Path: utzoo!utgpu!watserv1!watmath!uunet!elroy.jpl.nasa.gov!sdd.hp.com!uakari.primate.wisc.edu!caen!uwm.edu!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: D.melanogaster Arg-tRNA-2 gene. Message-ID: Date: 1 Mar 91 13:00:55 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 45 Approved: lear@genbank.bio.net Checksum: 57084 3 LOCUS DROTGR 133 bp ds-DNA INV 01-MAR-1991 DEFINITION D.melanogaster Arg-tRNA-2 gene. ACCESSION V00240 M25953 KEYWORDS transfer RNA; transfer RNA-Arg. SOURCE D.melanogaster DNA, clone pYH48 [J. Biol. Chem. 254, 10290-10294 (1979)],[Nucleic Acids Res. 10, 5393-5406 (1982)]. ORGANISM Drosophila melanogaster Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta; Pterygota; Neoptera; Holometabola; Diptera; Brachycera; Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae. REFERENCE 1 (bases 1 to 110) AUTHORS Silverman,S., Schmidt,O., Soell,D. and Hovemann,B. TITLE The nucleotide sequence of a cloned Drosophila arginine tRNA gene and its in vitro transcription in Xenopus germinal vesicle extracts JOURNAL J. Biol. Chem. 254, 10290-10294 (1979) STANDARD simple staff_review REFERENCE 2 (bases 1 to 112) AUTHORS Sharp,S., Dingermann,T. and Soell,D. TITLE The minimum intragenic sequences required for promotion of eukaryotic tRNA gene transcription JOURNAL Nucleic Acids Res. 10, 5393-5406 (1982) STANDARD simple staff_review REFERENCE 3 (sites) AUTHORS Dingermann,T. and Nerke,K. TITLE Primer extension analysis of tRNA gene transcripts synthesized in vitro and in vivo JOURNAL Anal. Biochem. 162, 466-475 (1987) STANDARD full staff_review COMMENT [J. Biol. Chem. 254, 10290-10294 (1979)] corresponds to EMBL (release 3.0) entry , dated 29-AUG-1983. FEATURES Location/Qualifiers misc_RNA 37..125 /note="Arg-tRNA precursor start" misc_RNA 40..125 /note="Arg-tRNA precursor start" tRNA 44..116 /anticodon=(pos:77..79,aa:Arg) /note="Arg-tRNA-2" BASE COUNT 28 a 30 c 33 g 42 t ORIGIN 67 bp upstream of HinfI site; chromosome 2R, region 42A. 1 ttgactttct acttgtttga actgttacac tcgcacgtca agcggtcctg tggcgcaatg 61 gataacgcgt ctgactacgg atcagaagat tccaggttcg actcctggca ggatcgaatt 121 tttttggcgt tat //