Path: utzoo!utgpu!watserv1!watmath!uunet!munnari.oz.au!yoyo.aarnet.edu.au!sirius.ucs.adelaide.edu.au!f.adelaide.edu.au!cthomas From: cthomas@f.adelaide.edu.au Newsgroups: bionet.molbio.methds-reagnts Subject: Help: RNA extraction from Gram Positives Message-ID: <1991Mar4.135643.1@f.adelaide.edu.au> Date: 4 Mar 91 04:26:43 GMT Sender: news@ucs.adelaide.edu.au Organization: University of Adelaide Lines: 15 Nntp-Posting-Host: f.adelaide.edu.au Hi, Does anyone have a reliable protocol for extraction of total RNA/mRNA from Gram positive bacteria like Bacillus and Listeria. Most of the the published methods I have seen apply to easily lysable Gram negative bacteria or eucaryotic cells. Thanks in advance, Connor Thomas Department of Microbiology and Immunology University of Adelaide South Australia Email: cthomas@f.adelaide.edu.au ------------------------------------------------------------------------------