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From: kim@m44.unm.edu
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Help: RNA extraction from Gram Positives
Message-ID: <009451A8.8B516520@m44.unm.edu>
Date: 4 Mar 91 19:54:48 GMT
References: <1991Mar4.135643.1@f.adelaide.edu.au>
Reply-To: kim@m44.unm.edu
Organization: UNM Cancer Center, Albuquerque, NM
Lines: 20

In article <1991Mar4.135643.1@f.adelaide.edu.au>, cthomas@f.adelaide.edu.au writes:
>Hi,
>Does anyone have a reliable protocol for extraction of total RNA/mRNA  from
>Gram positive bacteria like Bacillus and Listeria.  Most of the the
>published methods I have seen apply to easily lysable Gram negative bacteria
>or eucaryotic cells.
>
>Thanks in advance,
>
>Connor Thomas
>Department of Microbiology and Immunology
>University of Adelaide
>South Australia
>Email: cthomas@f.adelaide.edu.au
>
>------------------------------------------------------------------------------
In Analytical Biochemistry 176:464-467 [1989], there is a procedure for
preparing bacterial DNA using DMSO to lyse the cells.  It may be of use in your
application.  I have never used the method myself.
Daniel Kim