Path: utzoo!utgpu!watserv1!watmath!uunet!elroy.jpl.nasa.gov!sdd.hp.com!news.cs.indiana.edu!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Synthetic alpha-amylase gene, 5' end. Message-ID: Date: 13 Mar 91 13:03:39 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 29 Approved: lear@genbank.bio.net Checksum: 03913 2 LOCUS SYNAMYLS 120 bp ds-DNA SYN 13-MAR-1991 DEFINITION Synthetic alpha-amylase gene, 5' end. ACCESSION M62639 KEYWORDS alpha-amylase. SOURCE Synthetic DNA from B.licheniformis and B.stearothermophilus. ORGANISM Artificial gene Artificial sequences; Genes. REFERENCE 1 (bases 1 to 120) AUTHORS Jorgensen,P.L., Hansen,C.K., Poulsen,G.B. and Diderichsen,B. TITLE In vivo genetic engineering: homologous recombination as a tool for plasmid construction JOURNAL Gene 96, 37-41 (1990) STANDARD full staff_entry FEATURES Location/Qualifiers CDS 22..>120 /note="fusion gene" /product="alpha-amylase" /codon_start=22 sig_peptide 22..108 /product="alpha-amylase" /codon_start=22 mat_peptide 109..>120 /product="alpha-amylase" /codon_start=109 BASE COUNT 28 a 32 c 30 g 30 t ORIGIN 1 cattgaaagg ggaggagaat catgaaacaa caaaaacggc tttacgcccg attgctgacg 61 ctgttatttg cgctcatctt cttgctgcct cattctgcag cagcggcggc cgcaccgttt //