Path: utzoo!utgpu!watserv1!watmath!uunet!decwrl!mcnc!uvaarpa!murdoch!biochsn.acc.Virginia.EDU!wrp
From: wrp@biochsn.acc.Virginia.EDU (William R. Pearson)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Vertical agarose electrophoresis
Message-ID: <1991Mar11.183704.11883@murdoch.acc.Virginia.EDU>
Date: 11 Mar 91 18:37:04 GMT
References: <9103100855.AA04653@genbank.bio.net>
Sender: usenet@murdoch.acc.Virginia.EDU
Organization: University of Virginia
Lines: 23

In article <9103100855.AA04653@genbank.bio.net> EBMV36@ercvax.edinburgh.ac.uk writes:
>Is there anyone who has the experience of doing genomic southern hybridization
>using vertical electrophoresis?  According to the adv. of Stratagene, they
>claimed the vertical electrophoresis gives better resolution and saves the
>running time.  I am wondering why there is just only one model of commercial
>electrophoresis apparatus available if the vertical agarose electrophoresis
>has such wonderful magics.
>Any comment and discussion would be appreciated.
>
>Chao-Liang Wu
>Dept. of Molecular Biology
>Univ. of Edinburgh
>EBMV36@ercvax.edinburgh.ac.uk

	I am a great fan of vertical gel electrophoresis for Northern
and Southern blots.  I believe that it provides a quality and reproducibility
that is difficult to match with horizontal apparatus.  We use gel boxes
made be Watson Products, PO Box 247, (818) 797-5773.  They are a "mom &
pop" company that started making vertical gel apparatus just as agarose
was being developed for DNA fractionation.  They sell frosted (sand-blasted)
plates, which are essential for holding low-percentage gels in position.

Bill Pearson