Path: utzoo!news-server.csri.toronto.edu!utgpu!watserv1!watmath!uunet!bionet!UCSD.EDU!mwsmith
From: mwsmith@UCSD.EDU (Mike Smith)
Newsgroups: bionet.molbio.methds-reagnts
Subject: (none)
Message-ID: <9103140043.AA14252@sdchemd1.ucsd.edu>
Date: 14 Mar 91 00:43:14 GMT
Sender: daemon@genbank.bio.net
Lines: 24


     Has anyone tried the Strataclean resin from Stratagene?  Today I used 
this resin in a alkaline lysis miniprep method.  Basically the resin is used
to bind up protein from the cellular extract instead of a phenol chloroform 
extraction.  Stratagene claims that the miniprep DNA is suitable for digestions
and sequencing.  The miniprep DNA did cut with SstI, but I wonder if the 
background RNA will interfere with sequencing.
*****I have several questions for the BB:  *****
1) Has DNA purified using this resin worked for sequencing in anyones hands??
2) Is Stratagene the only source?  The resin is priced at $0.55-0.77/miniprep.
     Seems like a steep price for a reagent that they probably can make 
     by the bucketful, and later sell in 3ml and 9ml packages.
    
    I have no financial connection to the manufacturer.  Just looking for a 
less toxic approach to isolating DNA.

Mike 
        ---------------- Michael W. Smith ----------------
        |       Center for Molecular Genetics            |
	|       9500 Gilman Drive                        |
	|       University of California, San Diego      |
        |       La Jolla, CA  92093-0634                 |
        -------------- mwsmith@ucsd.bitnet ---------------
This is the size BB message I like to get.  Its short, sweet, and fits on a page