Path: utzoo!utgpu!watserv1!watmath!uunet!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: O.fallax macronuclear (81-MAC family, version A) DNA, region Gp-a. Message-ID: Date: 16 Mar 91 13:00:16 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 65 Approved: lear@genbank.bio.net Checksum: 46160 4 LOCUS OFA81MAB 397 bp ds-DNA INV 16-MAR-1991 DEFINITION O.fallax macronuclear (81-MAC family, version A) DNA, region Gp-a. ACCESSION M13034 KEYWORDS 81-MAC chromosome family; alternative DNA processing; homolog of mitochondrial solute carriers. SOURCE O.fallax (subkaryonide 3.5) macronuclear DNA, region Gp-a, clone pMA81. ORGANISM Oxytricha fallax Eukaryota; Animalia; Metazoa; Ciliophora; Polyhymenophora; Spirotricha; Hypotrichida; Sporadotrichina; Oxytrichidae. REFERENCE 1 (bases 1 to 397) AUTHORS Herrick,G. JOURNAL Unpublished (1987) Univ. of Utah, Salt Lake City, Utah 84132 STANDARD full staff_entry REFERENCE 2 (bases 1 to 397) AUTHORS Herrick,G., Hunter,D., Williams,K. and Kotter,K. TITLE Alternative processing during development of a macronuclear chromosomal family in Oxytricha fallax JOURNAL Genes Dev. 1, 1047-1058 (1987) STANDARD full staff_review REFERENCE 3 (bases 1 to 397) AUTHORS Herrick,G., Cartinhour,S.W., Williams,K.R. and Kotter,K.P. TITLE Multiple sequence versions of the Oxytricha fallax 81-MAC alternate processing family JOURNAL J. Protozool. 34, 429-434 (1987) STANDARD full staff_review COMMENT Draft entry and computer-readable sequence for [J. Protozool. 34, 429-434 (1987)] kindly provided by G.Herrick, 12-DEC-1986. This sequence is referred to as Gp-a in [J. Protozool. 34, 429-434 (1987)]. The 81-MAC chromosome family is created by alternative processing of the micronuclear DNA. The 81-MAC family consists of three sizes of macronuclear chromosomes: 4.9 kb (MACI), 2.9 kb (MACII), and 1.6 kb (MACIII). Alternative processing results from alternative choice of chromosome break/telomere-addition sites. The 1.6 kb region in common between the three chromosomes bears a transcribed open reading frame, the conceptual translation product of which is homologous to solute carrier proteins from plants and metazoans. There are three distinct micronuclear loci (sequence versions),vA, vB, and vC. Alternative processing of each micronuclear version independently gives rise to a I+II+III family of macronuclear chromosomes. The open reading frame is conserved in all three versions. Little is known about the structure of vB micronuclear DNA (no clones), but vA and vC micronuclear DNAs bear micronucleus-limited sequences called internal eliminated sequences (IESs). vA and vC have small IESs in common, but in addition, vC bears two large transposons, TBE1-1 and -2, which also are IESs. IESs are precisely excised, leaving one short flanking direct repeat in macronuclear DNA. The telomeric sequence ('g4t4)4 g4t' is added to position 397. FEATURES Location/Qualifiers BASE COUNT 125 a 40 c 72 g 158 t 2 others ORIGIN Unreported. 1 ttattctatt catatttgct ccaaggcctg agttagtgtg ttmcatagtt tgattgtaaa 61 ttaatagatg cactactctt gtttgaagat tcttttatag gtggagagta cgaargatta 121 gaattgttct aagctgatga ttgagaagaa gttattgtta tgataggcca ttttcatatt 181 ttaaatgaaa gaattgcctg tagattggta ggaattattg tttgaaagac cttaagacac 241 tttaagtggt taggtctatg gaataagata tttatttgtt tgcatttaac ttcctaactg 301 agaaagtttc taggtctaag attgttaaga ttatggataa ttcatttttg tttagaaata 361 ttttaaattc aaatttataa ctactataac tacacat //