Path: utzoo!utgpu!watserv1!watmath!uunet!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: O.fallax micronuclear (81-MAC family, version A) DNA, region Message-ID: Date: 16 Mar 91 13:00:17 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 69 Approved: lear@genbank.bio.net Checksum: 22699 4 LOCUS OFA81MAC1 92 bp ds-DNA INV 16-MAR-1991 DEFINITION O.fallax micronuclear (81-MAC family, version A) DNA, region SRRHGKH-a (partial). ACCESSION M15836 M13033 KEYWORDS 81-MAC chromosome family; alternative DNA processing; homolog of mitochondrial solute carriers. SEGMENT 1 of 3 SOURCE O.fallax (subkaryonide 3.5) micronuclear DNA, region SRRHGKH-a, clone L47-mic21. ORGANISM Oxytricha fallax Eukaryota; Animalia; Metazoa; Ciliophora; Polyhymenophora; Spirotricha; Hypotrichida; Sporadotrichina; Oxytrichidae. REFERENCE 1 (bases 1 to 92) AUTHORS Herrick,G. JOURNAL Unpublished (1987) Univ. of Utah, Salt Lake City, Utah 84132 STANDARD full staff_entry REFERENCE 2 (bases 1 to 92) AUTHORS Herrick,G., Hunter,D., Williams,K. and Kotter,K. TITLE Alternative processing during development of a macronuclear chromosomal family in Oxytricha fallax JOURNAL Genes Dev. 1, 1047-1058 (1987) STANDARD full staff_entry REFERENCE 3 (bases 1 to 92) AUTHORS Herrick,G., Cartinhour,S.W., Williams,K.R. and Kotter,K.P. TITLE Multiple sequence versions of the Oxytricha fallax 81-MAC alternate processing family JOURNAL J. Protozool. 34, 429-434 (1987) STANDARD full staff_review COMMENT Draft entry and computer-readable sequence for [J. Protozool. 34, 429-434 (1987)] kindly provided by G.Herrick, 12-DEC-1986. This sequence is the first 92 bp of that referred to as SRRHGKH-a in [J. Protozool. 34, 429-434 (1987)]. It also could be referred to as mic-SR-a by the conventions in [J. Protozool. 34, 429-434 (1987)] The 81-MAC chromosome family is created by alternative processing of the micronuclear DNA. The 81-MAC family consists of three sizes of macronuclear chromosomes: 4.9 kb (MACI), 2.9 kb (MACII), and 1.6 kb (MACIII). Alternative processing results from alternative choice of chromosome break/telomere-addition sites. The 1.6 kb region in common between the three chromosomes bears a transcribed open reading frame, the conceptual translation product of which is homologous to solute carrier proteins from plants and metazoans. There are three distinct micronuclear loci (sequence versions),vA, vB, and vC. Alternative processing of each micronuclear version independently gives rise to a I+II+III family of macronuclear chromosomes. The open reading frame is conserved in all three versions. Little is known about the structure of vB micronuclear DNA (no clones), but vA and vC micronuclear DNAs bear micronucleus-limited sequences called internal eliminated sequences (IESs). vA and vC have small IESs in common, but in addition, vC bears two large transposons, TBE1-1 and -2, which also are IESs. IESs are precisely excised, leaving one short flanking direct repeat in macronuclear DNA. Only one copy of the tandem repeat (positions 56-69) is found in vB and vC. FEATURES Location/Qualifiers repeat_region 56..62 /note="direct tandem repeat tgatata copy A" repeat_region 63..69 /note="direct tandem repeat tgatata copy B" BASE COUNT 39 a 6 c 15 g 32 t ORIGIN 1 aaaaagcgag aggtattatt gaatgaataa tcaagagttt atcaaagatt tagaatgata 61 tatgatataa tgctttataa tgtactttat ca //