Path: utzoo!utgpu!watserv1!watmath!uunet!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: O.fallax micronuclear (81-MAC family, version A) DNA, region Message-ID: Date: 16 Mar 91 13:00:20 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 66 Approved: lear@genbank.bio.net Checksum: 04083 4 LOCUS OFA81MAC3 353 bp ds-DNA INV 16-MAR-1991 DEFINITION O.fallax micronuclear (81-MAC family, version A) DNA, region mic-KH-a. ACCESSION M13042 KEYWORDS 81-MAC chromosome family; alternative DNA processing; homolog of mitochondrial solute carriers. SEGMENT 3 of 3 SOURCE O.fallax (subkaryonide 3.5) micronuclear DNA, region mic-KH-a, clone L47-mic21. ORGANISM Oxytricha fallax Eukaryota; Animalia; Metazoa; Ciliophora; Polyhymenophora; Spirotricha; Hypotrichida; Sporadotrichina; Oxytrichidae. REFERENCE 1 (bases 1 to 353) AUTHORS Herrick,G. JOURNAL Unpublished (1987) Univ. of Utah, Salt Lake City, Utah 84132 STANDARD full staff_entry REFERENCE 2 (bases 1 to 353) AUTHORS Herrick,G., Cartinhour,S.W., Williams,K.R. and Kotter,K.P. TITLE Multiple sequence versions of the Oxytricha fallax 81-MAC alternate processing family JOURNAL J. Protozool. 34, 429-434 (1987) STANDARD full staff_review COMMENT Draft entry and computer-readable sequence for [J. Protozool. 34, 429-434 (1987)] kindly provided by G.Herrick, 12-DEC-1986. The 81-MAC chromosome family is created by alternative processing of the micronuclear DNA. The 81-MAC family consists of three sizes of macronuclear chromosomes: 4.9 kb (MACI), 2.9 kb (MACII), and 1.6 kb (MACIII). Alternative processing results from alternative choice of chromosome break/telomere-addition sites. The 1.6 kb region in common between the three chromosomes bears a transcribed open reading frame, the conceptual translation product of which is homologous to solute carrier proteins from plants and metazoans. There are three distinct micronuclear loci (sequence versions),vA, vB, and vC. Alternative processing of each micronuclear version independently gives rise to a I+II+III family of macronuclear chromosomes. The open reading frame is conserved in all three versions. Little is known about the structure of vB micronuclear DNA (no clones), but vA and vC micronuclear DNAs bear micronucleus-limited sequences called internal eliminated sequences (IESs). vA and vC have small IESs in common, but in addition, vC bears two large transposons, TBE1-1 and -2, which also are IESs. IESs are precisely excised, leaving one short flanking direct repeat in macronuclear DNA. When the IES is excised the 5' imperfectly duplicated sequence 'atta' remains in the macronuclear DNA. The sequence of the homologous macronuclear DNA can be found in a separate entry: M15835. FEATURES Location/Qualifiers repeat_region 138..141 /note="5' imperfectly duplicated sequence" iDNA 142..210 /note="IES" repeat_region 207..210 /note="3' imperfectly duplicated sequence" BASE COUNT 125 a 56 c 27 g 145 t ORIGIN 5 bp upstream of KpnI site; about 0.9 kb after segment 2. 1 ggtaccttct tctccagtac tcaactaaaa ataaatttta taaatcgatg attcgatatt 61 ttattgataa atttaccttc catttaagta ggcatctttc ttgattgctt attttaaaat 121 aattttaata actttatatt attgtaatca cattaagatt tatagtataa tttcaaagaa 181 tcattttaat ttccttatat tcaagcaata attatgttta ttacaaatta tttcaattaa 241 gttcattcaa aaataaatct ctgccacctt taaaatctat atttaggtta actcaagtct 301 tcaaacaaca cttctctgtg aagaatcact tcttaaaaca ccagttttct act //