Path: utzoo!utgpu!watserv1!watmath!uunet!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: O.fallax micronuclear (81-MAC family, version C) DNA, region Message-ID: Date: 16 Mar 91 13:00:21 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 101 Approved: lear@genbank.bio.net Checksum: 22317 6 LOCUS OFA81MCA1 846 bp ds-DNA INV 16-MAR-1991 DEFINITION O.fallax micronuclear (81-MAC family, version C) DNA, region mic-BSRRHG-c. ACCESSION M13035 M13029 M13036 KEYWORDS 81-MAC chromosome family; TBE1 telomere bearing element; alternative DNA processing; homolog of mitochondrial solute carriers; transposon. SEGMENT 1 of 3 SOURCE O.fallax (subkaryonide 3.5) micronuclear DNA, regions SRRHG-c, mic-RR-c and mic-Rg-c, clones L47-mic[10,20]. ORGANISM Oxytricha fallax Eukaryota; Animalia; Metazoa; Ciliophora; Polyhymenophora; Spirotricha; Hypotrichida; Sporadotrichina; Oxytrichidae. REFERENCE 1 (sites) AUTHORS Herrick,G., Cartinhour,S., Dawson,D., Ang,D., Sheets,R., Lee,A. and Williams,K. TITLE Mobile elements bounded by C-4-A-4 telomeric repeats in Oxytricha fallax JOURNAL Cell 43, 759-768 (1985) STANDARD full staff_review REFERENCE 2 (bases 1 to 846) AUTHORS Herrick,G. JOURNAL Unpublished (1987) Univ. of Utah, Salt Lake City, Utah 84132 STANDARD full staff_entry REFERENCE 3 (bases 1 to 846) AUTHORS Herrick,G., Cartinhour,S.W., Williams,K.R. and Kotter,K.P. TITLE Multiple sequence versions of the Oxytricha fallax 81-MAC alternate processing family JOURNAL J. Protozool. 34, 429-434 (1987) STANDARD full staff_review COMMENT [Cell 43, 759-768 (1985)] sites; telomere-bearing element description. Draft entry and computer-readable sequence for [J. Protozool. 34, 429-434 (1987)] kindly provided by G.Herrick, 12-DEC-1986. This sequence would be named 'mic-SRRg-c' by the conventions in [J. Protozool. 34, 429-434 (1987)]. Bases 1-494 come from region SRRHG-c, 372-617 from mic-RR-c, and 612-846 from mic-Rg-c as referred to in [J. Protozool. 34, 429-434 (1987)]. The 81-MAC chromosome family is created by alternative processing of the micronuclear DNA. The 81-MAC family consists of three sizes of macronuclear chromosomes: 4.9 kb (MACI), 2.9 kb (MACII), and 1.6 kb (MACIII). Alternative processing results from alternative choice of chromosome break/telomere-addition sites. The 1.6 kb region in common between the three chromosomes bears a transcribed open reading frame, the conceptual translation product of which is homologous to solute carrier proteins from plants and metazoans. There are three distinct micronuclear loci (sequence versions),vA, vB, and vC. Alternative processing of each micronuclear version independently gives rise to a I+II+III family of macronuclear chromosomes. The open reading frame is conserved in all three versions. Little is known about the structure of vB micronuclear DNA (no clones), but vA and vC micronuclear DNAs bear micronucleus-limited sequences called internal eliminated sequences (IESs). vA and vC have small IESs in common, but in addition, vC bears two large transposons, TBE1-1 and -2, which also are IESs. IESs are precisely excised, leaving one short flanking direct repeat in macronuclear DNA. When TBE1-1 is excised one flanking 'aat' probably remains in macronuclear DNA, as judged from oligomer hybridization experiments. FEATURES Location/Qualifiers repeat_region 490..494 /note="5' IES duplicated sequence" iDNA 495..566 /note="IES" repeat_region 562..566 /note="3' IES duplicated sequence" repeat_region 715..717 /note="5' TBE1-1 duplicated insertion target sequence" repeat_region 718..734 /note="telomere sequence of TBE1-1" repeat_region 718..795 /note="5' TBE1-1 inverted terminal repeat" transposon 718..>846 /note="TBE1-1 insertion element" BASE COUNT 293 a 100 c 155 g 298 t ORIGIN 86 bp upstream of SphI site. 1 agagacggag gaatgattga atgtgcaaaa tatatcttta agaatgaaca tgggtttatg 61 ggattttgga gagggttctc agcatgcagt gcaagagcag tatttgctaa ttctttcatg 121 tttgtggctt atgaatatgc ttagaaaaaa gcgagaggta ttattgaatg aatgatcgtg 181 gatttatcaa agtttttgaa tgatataatg ctttataatg tactttatca aagtaactta 241 agactgtgat attaatatga actaagatta tatataaatt taatattttt aaataggttt 301 aaataacaaa taaatctcta ctttaacaca aagcatgtga taatacttta tgttagagac 361 aatttatttc tgaattctca gataattgcg agattttctt ttcctgaagg atgagcagct 421 cccatacctc cagtagctgt agtataagta gcaactaatt ttttattgaa ggcatctgtg 481 tatggatcat gaatcataac gatttataaa attttggaaa aaatcataat ctaaaatttc 541 tgcttcatta taataagtta ttgaatatcg taatcgatac cactttgata gtggaatctc 601 tgtggttgcc agaattcttg atcatagtag ttttcgttat agtagtctac caagaattga 661 gaaagataac aaatcaagaa caatctgagg aagtaggctt cacctcctgc atagaatcaa 721 aaccccaaaa ccccttaagt agatggttta gtgtattgat ttgtagagaa tttgttaggg 781 gttggggtta ttaatagttt ttaatgtgga aatttatagt aaatgtattt aatttaatca 841 ctcaaa //