Path: utzoo!utgpu!watserv1!watmath!uunet!bionet!snorkelwacker.mit.edu!spool.mu.edu!sol.ctr.columbia.edu!emory!wuarchive!rex!ukma!aunro!alberta!herald.usask.ca!ccu.umanitoba.ca!frist
From: frist@ccu.umanitoba.ca
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: (none)
Message-ID: <1991Mar14.151231.7722@ccu.umanitoba.ca>
Date: 14 Mar 91 15:12:31 GMT
References: <9103140043.AA14252@sdchemd1.ucsd.edu>
Organization: University of Manitoba, Winnipeg, Canada
Lines: 22

In article <9103140043.AA14252@sdchemd1.ucsd.edu> mwsmith@UCSD.EDU (Mike Smith) writes:
>
>     Has anyone tried the Strataclean resin from Stratagene?  Today I used 

I haven't tried it, but to answer your next question...

>and sequencing.  The miniprep DNA did cut with SstI, but I wonder if the 
>background RNA will interfere with sequencing.
I do all my DNA sequencing from phenol/CHCl3 extracted miniprep dsDNA, with
the E.coli RNA still there. The presence of RNA doesn't  appear to matter,
as I can usually read ~400 bases in a given reaction. However, I would like
to switch to something like Strataclean myself.

===============================================================================
Brian Fristensky                |  
Department of Plant Science     |              
University of Manitoba          |
Winnipeg, MB R3T 2N2  CANADA    |                THIS is THIS! 
frist@ccu.umanitoba.ca          |
Office phone:   204-474-6085    |
FAX:            204-275-5128    | 
===============================================================================