Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!zaphod.mps.ohio-state.edu!sdd.hp.com!uakari.primate.wisc.edu!crdgw1!uunet!biosys!ldow From: ldow@biosys.apldbio.COM (Leslie Johnston-Dow) Newsgroups: bionet.general Subject: pUC mutants? Keywords: pUC, mutants, help!! Message-ID: <147@biosys.apldbio.COM> Date: 22 Mar 91 00:11:50 GMT Organization: Applied Biosystems, Inc., Foster City, CA Lines: 14 Hello BioNetters, A colleague of mine believes she may have a problem concerning a mutant of pUC18 or 19. Specifically, that a single base change has occurred at the 3' end of the polylinker. We vaguely remember a paper describing such a mutation but cannot exactly place it. Does anyone know of such a mutation and/or paper? Thanks in advance! Leslie Johnston-Dow Applied Biosystems Inc. ldow@apldbio.com -- Leslie Johnston-Dow ldow@apldbio.com