Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!usc!ucselx!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Synthetic tac-lac promoter of the bacterial cloning vectors pMMB190 Message-ID: Date: 20 Mar 91 13:01:38 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 33 Approved: lear@genbank.bio.net Checksum: 02933 2 LOCUS TACLAC 192 bp ds-DNA SYN 20-MAR-1991 DEFINITION Synthetic tac-lac promoter of the bacterial cloning vectors pMMB190 and pMMB206. ACCESSION M37848 KEYWORDS . SOURCE Cloning vectors pMMB190 and pMMB206 DNA. ORGANISM Cloning vector Artificial sequences; Cloning vehicles. REFERENCE 1 (bases 1 to 192) AUTHORS Morales,V.M., Baeckman,A. and Bagdasarian,M. TITLE A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants JOURNAL Gene 97, 39-47 (1991) STANDARD simple staff_entry COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)] kindly submitted by V.M.Morales, 21-AUG-1990. Natl Res Council, Canada Plant Biotech Inst Saskatoon, Sask. S7N OW9 Canada FEATURES Location/Qualifiers promoter 11..54 /gene="tac" promoter 103..131 /gene="lacUV5" BASE COUNT 59 a 36 c 43 g 54 t ORIGIN 1 aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg 61 agcggataac aatttcacac aggaaactag gcaccccagg ctttacactt tatgcttccg 121 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac 181 catgattacg aa //