Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!usc!apple!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Bacteriophage Mu wild type DNA fragment with a gyrase cleavage Message-ID: Date: 27 Mar 91 13:01:25 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 30 Approved: lear@genbank.bio.net Checksum: 11134 2 LOCUS PMUCEN 150 bp ds-DNA PHG 27-MAR-1991 DEFINITION Bacteriophage Mu wild type DNA fragment with a gyrase cleavage site. ACCESSION M32302 KEYWORDS . SOURCE Bacteriophage Mu (wild type) DNA. ORGANISM Bacteriophage mu Viridae; ds-DNA nonenveloped viruses; Myoviridae. REFERENCE 1 (bases 1 to 150) AUTHORS Pato,M.L., Howe,M.M. and Higgins,N.P. TITLE A DNA gyrase-binding site at the center of the bacteriphage Mu genome is required for efficient replicative transposition JOURNAL Unpublished (1990) In Press STANDARD full staff_review COMMENT Draft entry and computer-readable sequence for [Unpublished (1990) In Press] kindly submitted by M.L.Pato, 23-FEB-1990. FEATURES Location/Qualifiers mutation 44..44 /note="a in wt; g in Mu nuB103" misc_feature 49..50 /note="DNA gyrase cleavage site in complimentary strand" mutation 50..50 /note="g in wt; c in Mu nuB1" BASE COUNT 40 a 38 c 27 g 45 t ORIGIN Map position at 18.0 kb. 1 acgcgtcagc gccgctctga ggcaataaac agaatcaggc ataaaatcag ccgcacagat 61 tttttaaaac gcgccacggg atttttaaac cggtatttaa cggtgtatga atcccgtttt 121 atcttccttt cactttcttt ctccagtact //