Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!zaphod.mps.ohio-state.edu!sdd.hp.com!decwrl!ames!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: E.coli mini-F plasmid repE mutant gene. Message-ID: Date: 28 Mar 91 13:03:20 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 27 Approved: lear@genbank.bio.net Checksum: 06693 2 LOCUS FPLMREPE 72 bp ds-DNA BCT 28-MAR-1991 DEFINITION E.coli mini-F plasmid repE mutant gene. ACCESSION M63628 KEYWORDS replication initiation protein. SOURCE Mini-F plasmid from E.coli DNA. ORGANISM Plasmid F Prokaryota; Bacteria. REFERENCE 1 (bases 1 to 72) AUTHORS Kawasaki,Y., Wada,C. and Yura,T. TITLE Mini-F plasmid mutants able to replicate in the Absence of sigma-32 mutations in the repE coding region producing hyperactive initiator protein JOURNAL J. Bacteriol. 173, 1064-1072 (1991) STANDARD full staff_entry FEATURES Location/Qualifiers CDS <1..72 /note="mutant gene with transposon insertion" /product="replication initiator protein" /gene="repE" /codon_start=1 transposon 20..>72 /note="Tn3" BASE COUNT 19 a 15 c 20 g 18 t ORIGIN 1 cgcgatatca cttccatgag gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 61 ttttggtcat ga //