Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!usc!samsung!news.cs.indiana.edu!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: L.lactis insertion sequence IS981. Message-ID: Date: 29 Mar 91 13:01:14 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 64 Approved: lear@genbank.bio.net Checksum: 05662 4 LOCUS LACNISC 1222 bp ds-DNA BCT 29-MAR-1991 DEFINITION L.lactis insertion sequence IS981. ACCESSION M33933 KEYWORDS insertion sequence. SOURCE L.lactis (strain LM0230) DNA, clone pKM40. ORGANISM Lactococcus lactis Prokaryota; Bacteria; Firmicutes; Regular asporogenous rods; Lactobacillaceae. REFERENCE 1 (bases 1 to 1222) AUTHORS Polzin,K.M. and McKay,L.L. TITLE Identification, DNA sequence, and distribution of IS981, a new, high-copy-number insertion sequence in lactococci JOURNAL Appl. Environ. Microbiol. 57, 734-743 (1991) STANDARD full staff_entry COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)] kindly submitted by K.M.Polzin 27-APR-1990. University of Minnesota Dept. of Food Science 1334 Eckles Avenue, St. Paul, MN 55108 e-mail: kayla@molbio.cbs.umn.edu FEATURES Location/Qualifiers repeat_region 1..40 /note="5' inverted repeat" misc_signal 33..38 /note="-10 region" misc_binding 44..50 /note="ribosomal binding site (put.)" CDS 58..318 /note="ORF1" /codon_start=58 misc_signal 269..274 /note="-35 region" misc_signal 294..299 /note="-10 region" CDS 330..1169 /note="ORF2" /codon_start=330 repeat_region 1183..1222 /note="3' inverted repeat" BASE COUNT 442 a 233 c 206 g 341 t ORIGIN 132 bp upstream of PvuII site. 1 gatgttatcc ttaaatctta gagtcactat tgtataattt agacaaagga caaaaacatg 61 caaaaacgct actcaaaaga atttaaagaa acccttatcg ccttctatca ttctggtcaa 121 tccgtcaccc agctgtctaa agaatatgac gtggcccctg caacaattta taaatggata 181 gacctctact ctaaatctaa tgaaagctcc gtctctaaag ctgattttct agaattaaaa 241 agacaactgg ctaaagttaa ggaagaacga gacatcttaa aaaaagtatt gaccatattc 301 gccgagaaaa agaagtgagt gctgcggata tggctcaaac catacaaact ttagcactca 361 atgtcagact aagctgtcaa ctccttgatg ttcctgaatc aagttattat gaacggatta 421 accgacaccc atctaaaact caattaagga gacaatacct gtcactcaaa atttctcaac 481 tcttcaatgc taaccgagga atctatggtg ctcctaaaat tcatcatctt ctacttaaac 541 aaggggaaaa agtcgggtta aaactggtac agaagctaat gaagcaactt caactcaagt 601 ctgtagtcat taagaaattt aagcctggat actcactaag tgatcacatc aatcgaaaaa 661 atctcataca gactgaacct acaaagaaaa ataaggtttg gtcaaccgac attacttata 721 ttcctactca acaaggatgg gcttatctct caaccattat ggatcgttat actaaaaaag 781 tcattgcttg ggatttgggc aagcgaatga ctgtagaatt agtgcaaaga actttaaata 841 aggccattaa atcacaagac tatccagaag ctgttattct tcattctgac caaggaagcc 901 agtatacgag tctagagtat gaagagttgc ttaagtatta tgggatgact cactctttca 961 gtcgaagggg atacccttat cataatgcca gtcttgaatc ttggcatgga catttaaaaa 1021 gagagtgggt gtaccaattt aaatataaga actttgaaga agcctatcag agtattttct 1081 ggtacatcga agccttttat aattcaaaac gaatccatca aagtttaggg tatcttacac 1141 ctaatcaatt tgaaaaggta agtgcttaaa ataaatagat taaaattcta cgtttgttac 1201 tctaaaaact tgacttaacg tc //