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From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: K.aerogenes histidine utilization proteins hutC (3' end) and hutU
Message-ID: <Apr.6.05.00.07.1991.1583@genbank.bio.net>
Date: 6 Apr 91 13:00:07 GMT
Sender: root@genbank.bio.net
Distribution: bionet
Lines: 47
Approved: lear@genbank.bio.net
Checksum: 46583     3

LOCUS       KAEHUTCU      250 bp ds-DNA             BCT       06-APR-1991
DEFINITION  K.aerogenes histidine utilization proteins hutC (3' end) and hutU
            (5' end) genes.
ACCESSION   M19665
KEYWORDS    histidine utilization protein.
SOURCE      K.aerogenes DNA.
  ORGANISM  Klebsiella aerogenes
            Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
            Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Nieuwkoop,A.J., Baldauf,S.A., Hudspeth,M.E. and Bender,R.A.
  TITLE     Bidirectional promoter in hut(P) region of the histidine
            utilization (hut) operons from Klebsiella aerogenes
  JOURNAL   J. Bacteriol. 170, 2240-2246 (1988)
  STANDARD  full staff_entry
REFERENCE   2  (sites)
  AUTHORS   Osuna,R., Boylan,S.A. and Bender,R.A.
  TITLE     In vitro transcription of the histidine utilization (hutUH) operon
            from Klebsiella aerogenes
  JOURNAL   J. Bacteriol. 173, 116-123 (1991)
  STANDARD  full staff_review
COMMENT     Draft entry and computer-readable sequence for [J. Bacteriol. 170,
            2240-2246 (1988)] kindly provided
            by R.Bender, 08-JUL-1988.
FEATURES             Location/Qualifiers
     CDS             <1..81
                     /note="histidine utilization protein hutC (AA at 1)"
                     /codon_start=1
     mRNA            100..>250
                     /note="hut mRNA"
     misc_binding    100..120
                     /note="CAP-cAMP binding site"
     misc_feature    complement(129..157)
                     /note="Pc2 promoter"
     misc_feature    162..183
                     /note="operator"
     CDS             224..>250
                     /note="histidine utilization protein hutU"
                     /codon_start=224
BASE COUNT       63 a     68 c     55 g     64 t
ORIGIN      151 bp upstream of BstNI site.
        1 tcgacaaccc acatcgtctc ccacgcgcgc ctgctgttcc ccggtagccg atatcgtctg
       61 caggggcgct ttggctcctg atccacgctg gcgtcagcaa aacgtgattg ctgacgcaat
      121 ataacaaaat tgtatcattt ctgttaaatc ctggcttgcg catgcttgta tagacaagta
      181 tatgtatcta cgtaaacaac gtttattgtc aggagaaacc ccgatgtcgc aaagcaaata
      241 tcgccagctg
//