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From: kliman@mbcl.rutgers.edu
Newsgroups: bionet.molbio.methds-reagnts
Subject: Seeking alternatives to silanizing gel plates
Message-ID: <384.2803536d@mbcl.rutgers.edu>
Date: 10 Apr 91 22:03:25 GMT
Lines: 10


     We have found that repeated silanization of glass plates used for DNA
sequencing gels eventually leads to problems in pouring the gel, i.e., the gel
mix does not flow evenly and forms persistent bubbles.  While we can correct
this by overnight soaking in 10-20% NaOH, we would rather not leave large
volumes of NaOH out overnight on a regular basis.  And, of course, we would
prefer to decrease our exposure to silane..
    Has anyone else experienced similar problems?  We'd appreciate any 
suggested alternatives to silanization that could be used on a daily basis.
Thanks.