Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!zaphod.mps.ohio-state.edu!wuarchive!bcm!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Yeast (S.cerevisiae) CUP1 locus encoding copper chelatin and an Message-ID: Date: 20 Apr 1991 12:01:42 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 124 Approved: lear@genbank.bio.net Checksum: 53160 7 LOCUS YSCCUP1 2010 bp ds-DNA PLN 20-APR-1991 DEFINITION Yeast (S.cerevisiae) CUP1 locus encoding copper chelatin and an URF. ACCESSION K02204 M64045 KEYWORDS chelatin; copper chelatin; copper metallothionein; metallothionein; unidentified reading frame. SOURCE Yeast (S.cerevisiae: X2180 [Proc. Natl. Acad. Sci. U.S.A. 81, 337-341 (1984)]) DNA, clones YRp7 [Proc. Natl. Acad. Sci. U.S.A. 81, 337-341 (1984)] and YEp13 [Proc. Natl. Acad. Sci. U.S.A. 81, 3332-3336 (1984)]. ORGANISM Saccharomyces cerevisiae Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes; Endomycetales; Saccharomycetaceae. REFERENCE 1 (bases 1 to 1998) AUTHORS Karin,M., Najarian,R., Haslinger,A., Valenzuela,P., Welch,J. and Fogel,S. TITLE Primary structure and transcription of an amplified genetic locus: The CUP1 locus of yeast JOURNAL Proc. Natl. Acad. Sci. U.S.A. 81, 337-341 (1984) STANDARD full staff_review REFERENCE 2 (bases 1075 to 2010) AUTHORS Butt,T.R., Sternberg,E.J., Gorman,J.A., Clark,P., Hamer,D., Rosenberg,M. and Crooke,S.T. TITLE Copper metallothionein of yeast, structure of the gene, and regulation of expression JOURNAL Proc. Natl. Acad. Sci. U.S.A. 81, 3332-3336 (1984) STANDARD full staff_review REFERENCE 3 (bases 1075 to 2010) AUTHORS Butt,T.R. JOURNAL Unpublished (1985) Smith Kline & French Labs, Philadelphia, PA STANDARD full staff_review REFERENCE 4 (sites) AUTHORS Silar,P., Butler,G. and Thiele,D.J. TITLE Heat shock transcription factor activates transcription of the yeast metallothionein gene JOURNAL Mol. Cell. Biol. 11, 1232-1238 (1991) STANDARD full staff_review COMMENT Copper resistance in yeast is controlled by the CUP1 locus, which can be found in up to 15 tandemly iterated copies on chromosome VIII. The basic amplified CUP1 unit contains two distinct transcription units coding for the inducible copper binding protein, chelatin (61 aa), and an undetermined protein found at bases 329-1069 designated protein X (246 aa) in [Proc. Natl. Acad. Sci. U.S.A. 81, 337-341 (1984)]. Possible TATA boxes were found at bases 202 and 216 for URF-X, and bases 1392 and 1436 for the chelatin gene. Termination sequences were found at bases 1244 and 1260 for URF-X, and bases 1820 and 1837 for the chelatin gene [Proc. Natl. Acad. Sci. U.S.A. 81, 337-341 (1984)]. [Proc. Natl. Acad. Sci. U.S.A. 81, 3332-3336 (1984)] identifies a potential poly-adenylation signal starting at base 1981. Although the yeast copper binding protein and the metallothionein proteins both provide heavy metal binding, there are major differences between the proteins; [Proc. Natl. Acad. Sci. U.S.A. 81, 337-341 (1984)] suggests that the proteins are convergently related, and refers to the protein as copper chelatin; [Proc. Natl. Acad. Sci. U.S.A. 81, 3332-3336 (1984)] refers to it as copper metallothionein (Cu-MT). FEATURES Location/Qualifiers repeat_region 1..1998 /note="copper resistance repeat unit" mRNA 248..>1069 /note="ORF-X mRNA (alt.)" mRNA 257..>1069 /note="ORF-X mRNA (alt.)" promoter 1289..1378 /gene="CUP1" /citation=Mol. Cell. Biol. 11, 1232-1238 (1991) protein_bind 1289..1307 /note="HSF-binding site" /gene="CUP1" /citation=Mol. Cell. Biol. 11, 1232-1238 (1991) protein_bind 1327..1356 /note="HSF-binding site" /gene="CUP1" /citation=Mol. Cell. Biol. 11, 1232-1238 (1991) mRNA 1469..>1719 /note="chelatin mRNA (alt.)" mRNA 1475..>1719 /note="chelatin mRNA (alt.)" mRNA 1478..>1719 /note="chelatin mRNA (alt.)" CDS 1534..1719 /note="copper chelatin" /codon_start=1534 BASE COUNT 713 a 327 c 313 g 657 t ORIGIN 15 bp upstream of Fnu4HI site, chromosome VIII. 1 attcatggta cccgctgctg aaaacctatc tccgatacct gcctctattg atacgaacga 61 cattccttta attgctaacg atttaaaatt actggaaacg caagcaaaat tgataaatat 121 tctgcaaggt gttcctttct acttgccagt aaatttaacc aaaattgaaa gtctgttaga 181 aaccttgact atgggcgtga gtaatacagt agacttatat tttcatgaca acgaagtcag 241 aaaagaatgg aaagacactt taaattttat caataccatt gtttatacaa attttttcct 301 ttttgttcaa aacgaatcct ctttgtccat ggcagttcaa cattcttcta acaacaataa 361 gacctcgaac tctgaaagat gtgcaaagga tctgatgaaa attatttcta atatgcacat 421 tttttactca ataacattta attttatctt ccccataaag tcgataaagt cattttcaag 481 cggcaataat cgctttcatt ctaatggtaa agaattttta ttcgcaaatc attttattga 541 aatcttacag aattttatag caatcacatt tgctattttc caacgttgtg aagtaatatt 601 atatgacgaa ttttacaaaa atctttcaaa tgaggagatt aatgttcaat tgctattgat 661 tcatgacaag attttggaaa ttttaaaaaa aatagaaatt atcgtatcct ttttacgaga 721 tgaaatgaat agcaacggaa gtttcaaatc tattaaaggt ttcaacaagg ttttgaatct 781 gattaaatat atgctgagat ttagcaagaa aaaacaaaat tttgcgagaa actctgataa 841 caataatgtt acagattata gtcagtcggc gaagaacaaa aatgttctct tgaaattccc 901 cgttagtgaa ctgaacagaa tctatttaaa atttaaggag atttcagatt ttttaatgga 961 aagagaagtt gtccaaagga gtataattat tgacaaggat ttggaatctg ataatctggg 1021 tattactacg gcaaacttca acgatttcta tgatgcattt tataattagt aagccgatcc 1081 cattaccgac atttgggcgc tatacgtgca tatgttcatg tatgtatctg tatttaaaac 1141 acttttgtat tatttttcct catatatgtg tataggttta tacggatgat ttaattatta 1201 cttcaccacc ctttatttca ggctgatatc ttagccttgt tactagttag aaaaagacat 1261 ttttgctgtc agtcactgtc aagagattct tttgctggca tttcttctag aagcaaaaag 1321 agcgatgcgt cttttccgct gaaccgttcc agcaaaaaag actaccaacg caatatggat 1381 tgtcagaatc atataaaaga gaagcaaata actccttgtc ttgtatcaat tgcattataa 1441 tatcttcttg ttagtgcaat atcatataga agtcatcgaa atagatatta agaaaaacaa 1501 actgtacaat caatcaatca atcatcacat aaaatgttca gcgaattaat taacttccaa 1561 aatgaaggtc atgagtgcca atgccaatgt ggtagctgca aaaataatga acaatgccaa 1621 aaatcatgta gctgcccaac ggggtgtaac agcgacgaca aatgcccctg cggtaacaag 1681 tctgaagaaa ccaagaagtc atgctgctct gggaaatgaa acgaatagtc tttaatatat 1741 tcatctaact atttgctgtt tttaattttt aaaaggagaa ggaagtttaa tcgacgattc 1801 tactcagttt gagtacactt atgtattttg tttagatact ttgttaattt ataggtatac 1861 gttaataatt aagaaaagga aataaagtat ctccatatgt cgccccaaga ataaaatatt 1921 attaccaaat tctagtttgc ctaacttaca actctgtata gaatccccag atttcgaata 1981 aaaaaaaaaa aaaaagctat tcatggtacc //