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From: pollanen@csc.fi
Newsgroups: bionet.molbio.methds-reagnts
Subject: Cloning with PGEM -3Z and -4Z -HELP!!
Message-ID: <1991Apr26.135538.1@csc.fi>
Date: 26 Apr 91 13:55:38 GMT
Sender: usenet@nic.funet.fi
Lines: 9

HELLO

HAS ANYONE EXPERIENCE WITH PROMEGA's PGEM -3Z AND PGEM -4Z
CLONING VECTORS.... I HAVE TRIED TO LIGATE ABOUT 3.7-3.9Kb
INSERT INTO THEM BUT NOT SUCCEEDED WHAT THINGS DO I HAVE 
TO CHECK WHICH PROBABLY INHIBIT T4 DNA LIGASE ENZYME?


YOURS RAIMO POLLANEN(M.S.)