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From: bertheau@jouy.inra.fr (Yves Bertheau)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Genomic substraction
Message-ID: <9104270238.AA14321@genbank.bio.net>
Date: 26 Apr 91 16:30:32 GMT
Sender: daemon@genbank.bio.net
Lines: 13


We have some troubles during the course of a genomic substraction with ligation

Does somebody try ligation of oligonucleotides adaptators (one phosphorylated at
PCR using one strand of the adaptataors as a primer ?

We were trying to use Ausubel's protocol...

Any help would be appreciated.

Thanks in advance.

Yves Bertheau, Pathologie vegetale, INRA, PAris France.