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From: msw1633@summa.tamu.edu (WHITSITT, MARK STEVEN)
Newsgroups: sci.bio
Subject: Re: Quiz time
Message-ID: <15253@helios.TAMU.EDU>
Date: 24 Apr 91 17:15:57 GMT
References: <1991Apr24.134105.25827@pa.dec.com>
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In article <1991Apr24.134105.25827@pa.dec.com>, mikkelson@breakr.enet.dec.com (snopes) writes...
>..... for my own satisfaction I'd like to see how others
> would answer these simple multiple-choice questions:
> 
> A "high-energy bond"
> 
>  a) absorbs a large amount of free energy when the phosphate group is
>     attached during hydrolysis.
>  b) is formed when ATP is hydrolyzed to ADP and one phosphate group
>  c) is usually found in each glucose molecule; that is why glucose is
>     chosen as the starting point for glycolysis.
>  d) none of the above
> 
a) is wrong because a phosphate group is not attached in *hydrolysis*
b) is wrong because hydrolysis breaks bonds; it doesn't *form* them.
c) is wrong because the high energy bonds must be added by phosphorylation 
during glycolysis (ie F-6-P) in order to gain energy in the form of ATP.  These 
high energy bonds are not present in glucose per se.

--This leaves d) as the correct answer.

>When NAD combines with hydrogen, the NAD is
> 
>  a) reduced  b) oxidized  c) phosphorylated  d) denatured  e) none of these
> 

Very simple.  NAD is the oxidized form which is subsequently *REDUCED* when 
combined with H to form NADH.

>The oxygen released in photosynthesis comes from
> 
>  a) carbon dioxide  b) glucose  c) ribulose biphosphate 
>  d) water  e) atmospheric oxygen
> 
the answer is d) water  (I'm pretty sure, but it's been a while :)   )

>Which of the following has the most energy?
> 
>  a) AMP  b) ADP  c) ATP  d) glucose  e) NADPH
> 
glucose has the "most energy" potential as more high energy bonds can be formed 
and subsequently utilized in ATP.  But looking at each individual molecule, The 
gamma Phosphate high energy bond probably makes it the one with the most usable 
physiological energy. NADPH, however, has more bonds overall (high energy or 
otherwise) and may be considered to be the most energetic.

This question is a very poorly worded question and would require that the person 
answering had heard the lecture in which the teacher explained his rationale and 
what exactly he meant by "the most energy"  Don't you agree?



Mark S. Whitsitt, N5RJF        Texas A&M University, Dept of Biochemistry
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