Path: utzoo!utgpu!news-server.csri.toronto.edu!bonnie.concordia.ca!uunet!zaphod.mps.ohio-state.edu!rpi!uwm.edu!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Yeast enolase 1 [phosphopyruvate hydratase, EC 4.2.1.11] gene Message-ID: Date: 1 May 91 12:00:49 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 76 Approved: lear@genbank.bio.net Checksum: 08535 4 LOCUS YSCENO1 741 bp ds-DNA PLN 01-MAY-1991 DEFINITION Yeast enolase 1 [phosphopyruvate hydratase, EC 4.2.1.11] gene (ENO1), 5' end, and flank. ACCESSION D00410 M27568 M29128 KEYWORDS enolase 1; phosphopyruvate hydratase; promoter. SOURCE Saccharomyces cerevisiae (strain AH22) chromosomal DNA, clone pMC1587-ENO-C. ORGANISM Saccharomyces cerevisiae Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes; Endomycetales; Saccharomycetaceae. REFERENCE 1 (bases 1 to 741) AUTHORS Uemura,H., Jigami,Y., Tanaka,H., Toshimitu,N., Paterson,M. and Nakasato,S. TITLE Nucleotide sequence of the 5' flanking region responsible for the enhancement of the expression of yeast enolase 1 gene JOURNAL J. Biochem. 98, 859-862 (1985) STANDARD full staff_entry REFERENCE 2 (sites) AUTHORS Uemura,H., Shiba,T., Paterson,M., Jigami,Y. and Tanaka,H. TITLE Identification of a sequence containing the positive regulatory region of Saccharomyces cerevisiae gene EN01 JOURNAL Gene 45, 67-75 (1986) STANDARD full staff_review COMMENT This sequence of ENO1 5' flanking region is very important for strong promoter function of ENO1. FEATURES Location/Qualifiers misc_feature 28..37 /note="palindromic structure" CDS 36..188 /note="ORF" /codon_start=36 misc_feature 77..84 /note="palindromic structure" misc_feature 82..89 /note="palindromic structure" repeat_region 158..191 /note="inverted repeat" CDS 188..265 /note="ORF" /codon_start=188 misc_feature 194..223 /note="AT rich stretch" TATA_signal 593..599 /gene="EN01" /citation=Gene 45, 67-75 (1986) mRNA (698.701)..741 /product="enolase 1" /gene="EN01" /citation=Gene 45, 67-75 (1986) /partial misc_feature 701..704 /note="CAAG box" /gene="EN01" /citation=Gene 45, 67-75 (1986) CDS 739..741 /product="enolase 1" /gene="EN01" /codon_start=739 /citation=Gene 45, 67-75 (1986) /partial BASE COUNT 220 a 188 c 133 g 200 t ORIGIN 1 bp upstream of EcoRI site. 1 ctgcagcttt tgcccgggcg gtcattgatg catgcatgtg ccgtgaagcg ggacaaccag 61 aaaagtcgtc tataaatgcc ggcacgtgcg atcatcgtgg cggggtttta agagtgcata 121 tcacaaattg tcgcattacc gcggaaccgc cagatattca ttacttgacg caaaagcgtt 181 tgaaataatg acgaaaaaga aggaagaaaa aaaaagaaaa ataccgcttc taggcgggtt 241 atctactgat ccgagcttcc actaggatag cacccaaaca cctgcatatt tggacgacct 301 ttacttacac caccaaaaac cactttcgcc tctcccgccc ctgataacgt ccactaattg 361 agcgattacc tgagcggtcc tcttttgttt gcagcatgag acttgcatac tgcaaatcgt 421 aagtagcaac gtctcaaggt caaaactgta tggaaacctt gtcacctcac ttaattctag 481 ctagcctacc ctgcaagtca agaggtctcc gtgattccta gccacctcaa ggtatgcctc 541 tccccggaaa ctgtggcctt ttctggcaca catgatctcc acgatttcaa catataaata 601 gcttttgata atggcaatat taatcaaatt tattttactt ctttcttgta acatctctct 661 tgtaatccct tattccttct agctattttt cataaaaaac caagcaactg cttatcaaca 721 cacaaacact aaatcaaaat g //