Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!zaphod.mps.ohio-state.edu!wuarchive!uunet!bionet!ENZYME.BERKELEY.EDU!pquail
From: pquail@ENZYME.BERKELEY.EDU (Peter Quail)
Newsgroups: bionet.genome.arabidopsis
Subject: Biolistic Transformations
Message-ID: <9105061943.AA14144@enzyme>
Date: 6 May 91 19:43:28 GMT
Sender: daemon@genbank.bio.net
Lines: 9


In response to Chris Town's question about bombarding Arabidopsis, I have 
used the Dupont Biolistic gun for this purpose.  I used a standard DNA
and particle preparation protocol and shot 2, 3, and 4 day old etiolated
seedlings as well as 3-week old green plants.  The 2 day seedlings worked
somewhat better than the 3 or 4 day old ones.  I did not try earlier 
stages.  Feel free to contact me directly (415-559-5916) for further
details.
Tim Caspar