Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!crdgw1!uunet!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Yeast (S.cerevisiae) tau repetitive element and Cys-tRNA. Message-ID: Date: 4 May 91 12:00:08 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 77 Approved: lear@genbank.bio.net Checksum: 59860 5 LOCUS YSCTAU2 1354 bp ds-DNA PLN 04-MAY-1991 DEFINITION Yeast (S.cerevisiae) tau repetitive element and Cys-tRNA. ACCESSION K02653 M64789 KEYWORDS pseudogene; repetitive sequence; transfer RNA; transfer RNA-Cys. SEGMENT 2 of 2 SOURCE Yeast (S.cerevisiae; wild-type strain M1417-1c) DNA, clone pFG26 (derivative of pFG203). ORGANISM Saccharomyces cerevisiae Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes; Endomycetales; Saccharomycetaceae. REFERENCE 1 (bases 1 to 1354) AUTHORS Genbauffe,F.S., Chisholm,G.E. and Cooper,T.G. TITLE Tau, Sigma, and Delta: A family of repeated elements in yeast JOURNAL J. Biol. Chem. 259, 10518-10525 (1984) STANDARD full staff_review REFERENCE 2 (sites) AUTHORS Jentsch,S. TITLE A pseudogene for a novel ubiquitn C-terminal hydrolase of S.cerevisiae JOURNAL Nucleic Acids Res. 19, 1147-1147 (1991) STANDARD full staff_review COMMENT A 2.6 kb BgII-EcoRI fragment upstream of the DUR1,2 gene contained a new repeated DNA element, tau. Tau is flanked by two directly repeated sequences which themselves flank an inverted repeat. The sequence organization of tau is very similar to that observed at the termini of the delta and sigma repetitive elements indicating that tau, sigma, and delta probably belong to the same family [J. Biol. Chem. 259, 10518-10525 (1984)]. The inverted repeat is identical to those reported in transposable elements, suggesting that tau may be capable of transposition either alone or in association with some larger element [J. Biol. Chem. 259, 10518-10525 (1984)]. The 5 bp insertion target sequence was different for the two plasmids isolated by [J. Biol. Chem. 259, 10518-10525 (1984)], 'tatta' in pFG26 and 'gaatc' in pFG106. FEATURES Location/Qualifiers tRNA complement(173..244) /anticodon=(pos:211..213,aa:Cys) /note="Cys-tRNA" CDS 248..513 /pseudo /product="ubiquitin C-terminal hydrolase" /codon_start=248 /citation=Nucleic Acids Res. 19, 1147-1147 (1991) misc_feature complement(649..653) /note="tau insertion site target sequence, 3' copy" repeat_region complement(654..1024) /note="tau repetitive element" misc_feature complement(1025..1029) /note="tau insertion site target sequence, 5' copy" BASE COUNT 456 a 238 c 243 g 417 t ORIGIN About 2.5 kb after segment 1; 1 bp upstream of BglII site. 1 agatctgcca attttaaaaa gggtattgcc aatacacata gtagccttat ctgcagcaag 61 ccataggact aatgtgttcg acgtcgttgg ggaaaaaaag caaagaacat gttgcctttg 121 aggagactgc agcaagaaaa aaagttcaat aaggattgca aaaaaaaaaa gaagctcgca 181 ctcaggatcg aactaaggac caacagattt gcaatctgct gcgctaccac tgcgccatac 241 gagctttcta ttacatgtgg gaagactaat atagatagta tagtcaatta tacgaagtag 301 tgccagcccc acatataaga gaacggtgga atatttgaac tgtatgcaaa gaagtttcag 361 cggggacctt tattttagaa aaagcgaata aatgtcgctc atttgtttaa acggtatttg 421 ttggactgag ggttgcttcc atacggaaat gctaacgaag aggatgtgct gaattttgct 481 atgtttggaa tgggctcctc catctgggaa tgataatcat ttttattatt gtgtgttcaa 541 atacacttac acatctgttt tttcgtggta aggaatccga gaatcacatg aggtttggga 601 tagttgctgg caatcaatga atgacatcag ataaaacgaa aggagaaata atatgttgat 661 aattagaggt taaaaattag tattaatgaa gaaataatta ctgatcttct tatactaaat 721 aagagaggta tataaaacac acgccgattg gtcatattaa tcatgaccaa tataatagtg 781 attccggtag ttactataca ttgatgtgac gactcatatt cctcatatat gtacctacca 841 taacatgttc aactaatagg tctttaacac agcttcagta ttgtctgagc ttctcgttta 901 acattccttc tgcaataggc gcaatcacac ttaaacgtat acgagttgta cattaatata 961 cgatgtaagc attgaattgt taccatagca actcatgtca ctattaatta ctctcgttcc 1021 aacataatat tattatggag taatatctat tccctttcgt ggattccaag attctcgagg 1081 tatatttcca gtatattttg tatacctgat attatagcct ttagcaacaa cgaaatcaac 1141 atttatctca aatttcacct gtttctcatt tttaagcgcc tagcccatct aatcatctta 1201 actattcatc agggaaaatt aatatacatt gtgctcttaa tgtttcgaaa aataatcaaa 1261 atcaatagaa atagcgtttt gctactaaca aagccctttg atccaaagtt ggcttctgag 1321 aacttaacat ttcagtagaa caagcctcaa gctt //