Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!dali.cs.montana.edu!uakari.primate.wisc.edu!caen!uwm.edu!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Mu inserted into plasmid lambda-plac5 lac Z gene, 3' junction. Message-ID: Date: 8 May 91 12:01:30 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 27 Approved: lear@genbank.bio.net Checksum: 32674 2 LOCUS PMUECJ2 68 bp ds-DNA PHG 08-MAY-1991 DEFINITION Mu inserted into plasmid lambda-plac5 lac Z gene, 3' junction. ACCESSION M10802 KEYWORDS recombination junction. SEGMENT 2 of 2 SOURCE Mu DNA and plasmid lambda-plac5 DNA, clone Bu8098. ORGANISM Bacteriophage mu Viridae; ds-DNA nonenveloped viruses; Myoviridae. REFERENCE 1 (bases 1 to 68) AUTHORS Allet,B. TITLE Mu insertion duplicates a five base pair sequence at the host inserted site JOURNAL Cell 16, 123-129 (1979) STANDARD simple staff_review COMMENT Mu insertion seems to have a striking similarity to typical IS-mediated insertions that were found to be associated with a short DNA duplication at the target site. FEATURES Location/Qualifiers misc_recomb 38^39 /note="Mu DNA end/phage DNA start" repeat_region 39..43 /note="3' insertion target sequence" BASE COUNT 8 a 21 c 21 g 18 t ORIGIN 1 atcgtgaaac gctttcgcgt ttttcgtgcg ccgcttcact ggaagtcgcc gcgccactgg 61 tggtggcc //