Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!usc!wuarchive!uwm.edu!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Human salivary alpha-amylase (amy1) gene, exon 1. Message-ID: Date: 8 May 91 12:01:35 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 77 Approved: lear@genbank.bio.net Checksum: 25306 5 LOCUS HUMAMYA101 941 bp ds-DNA PRI 08-MAY-1991 DEFINITION Human salivary alpha-amylase (amy1) gene, exon 1. ACCESSION M18715 K02214 KEYWORDS alpha-amylase; amylase. SEGMENT 1 of 10 SOURCE Human salivary gland, cDNA to mRNA, clones pHSA7 and pHSA15 and [Gene 28, 263-270 (1984)],[Gene 41, 299-304 (1986)],[Gene 50, 371-372 (1986)] and Human cosmid library DNA, clone cosP2 [Gene 60, 57-64 (1987)]. ORGANISM Homo sapiens Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae. REFERENCE 1 (bases 543 to 931) AUTHORS Nakamura,Y., Ogawa,M., Nishide,T., Emi,M., Kosaki,G., Himeno,S. and Matsubara,K. TITLE Sequences of cDNAs for human salivary and pancreatic alpha- amylases JOURNAL Gene 28, 263-270 (1984) STANDARD full staff_review REFERENCE 2 (bases 1 to 941) AUTHORS Nishide,T., Nakamura,Y., Emi,M., Yamamoto,T., Ogawa,M., Takesada,M. and Matsubara,K. TITLE Primary structure of human salivary alpha-amylase gene JOURNAL Gene 41, 299-304 (1986) STANDARD full staff_review REFERENCE 3 (bases 543 to 931) AUTHORS Nishide,T., Emi,M., Nakamura,Y. and Matsubara,K. TITLE Corrected sequences of cDNAs for human salivary and pancreatic alpha-amylases JOURNAL Gene 50, 371-372 (1986) STANDARD full staff_review REFERENCE 4 (bases 1 to 941) AUTHORS Horii,A., Emi,M., Tomita,N., Nishide,T., Ogawa,M., Mori,T. and Matsubara,K. TITLE Primary structure of human pancreatic alpha-amylase gene: Its comparison with human salivary alpha-amylase gene JOURNAL Gene 60, 57-64 (1987) STANDARD full staff_entry COMMENT [Gene 50, 371-372 (1986)] revises [Gene 28, 263-270 (1984)]. [Gene 60, 57-64 (1987)] revises [Gene 28, 263-270 (1984)],[Gene 41, 299-304 (1986)],[Gene 50, 371-372 (1986)]. Draft entry and computer readable copy of sequence [Gene 28, 263-270 (1984)] kindly provided by A.Horii (14-MAR-1988). [Gene 28, 263-270 (1984)] also sequenced the pancreatic alpha-amylase mRNA. Comparison of the two alpha-amylase nucleotide sequences reveals 96% homology in the coding region. Comparison of the sequences of human alpha-amylase cDNAs with those for mouse suggests the possibility of gene conversion between the two genes of alpha-amylase. FEATURES Location/Qualifiers exon 764..931 /note="alpha-amylase, exon 1 /map='1p21' /hgml_locus_uid='LJ0263N'" intron 932..>941 /note="alpha-amylase, intron A" BASE COUNT 258 a 160 c 211 g 312 t ORIGIN 193 bp upstream of XbaI site; chromosome 1p21. 1 gcattcaagt taactcttcc ccttggtatc tgtacatacc tttgatgtca gtgtttagta 61 cacgtggctt ggtcacttca tggctaaaaa cgtgcttgtg gaagacaagt ctggcttggt 121 gagtctgtgt ggtcagcagt ctctgatccg tgcagggtat taatgtgtca gggctgagtg 181 ttctgagatt tatctagagg ctgggaaggg ctcctgaacc agttgtttcc gtcttgtcgg 241 tctgtcaggg ttggaaagtc caagccatag gacccagttt cctttcttag cttacgttat 301 ctaccagagc accgtgggct gttacttgcc ttgagttgga agcggttcgc atttataccg 361 gtaaatgtat tcatcctttt aatttatgta aagtttttta gtatgcaatt ctcgatcttt 421 taagagttga caacaaattt tggttttctg ctgttatgtg agaacattag gccacagcaa 481 catgtcattg tgtaaggaaa aataaaagtg ctaccatatg caaaaaaaaa aaaaaaaaga 541 aaagaaaaga aacattaatg tctaagaggt cattgagatg atttccatga gagacttttt 601 gatgttcttc accagttagg attattattg ataatccttt tcagattatg aataaacagt 661 ttgccctcaa gtatttattc atgctactat ttacattgta aaatgtgctt cttacaggaa 721 tataaatagt ttctggaaag gacactgaca acttcaaagc aaaatgaagc tcttttggtt 781 gcttttcacc attgggttct gctgggctca gtattcctca aatacacaac aaggacgaac 841 atctattgtt catctgtttg aatggcgatg ggttgatatt gctcttgaat gtgagcgata 901 tttagctccc aagggatttg gaggggttca ggtgggtatg a //