Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!uwm.edu!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Human salivary alpha-amylase (amy1) gene, exon 4. Message-ID: Date: 8 May 91 12:01:41 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 68 Approved: lear@genbank.bio.net Checksum: 58615 4 LOCUS HUMAMYA104 251 bp ds-DNA PRI 08-MAY-1991 DEFINITION Human salivary alpha-amylase (amy1) gene, exon 4. ACCESSION M18721 K02214 KEYWORDS alpha-amylase; amylase. SEGMENT 4 of 10 SOURCE Human salivary gland, cDNA to mRNA, clones pHSA7 and pHSA15 and [Gene 28, 263-270 (1984)],[Gene 41, 299-304 (1986)],[Gene 50, 371-372 (1986)] and Human cosmid library DNA, clone cosP2 [Gene 60, 57-64 (1987)]. ORGANISM Homo sapiens Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae. REFERENCE 1 (bases 11 to 241) AUTHORS Nakamura,Y., Ogawa,M., Nishide,T., Emi,M., Kosaki,G., Himeno,S. and Matsubara,K. TITLE Sequences of cDNAs for human salivary and pancreatic alpha- amylases JOURNAL Gene 28, 263-270 (1984) STANDARD full staff_review REFERENCE 2 (bases 1 to 251) AUTHORS Nishide,T., Nakamura,Y., Emi,M., Yamamoto,T., Ogawa,M., Takesada,M. and Matsubara,K. TITLE Primary structure of human salivary alpha-amylase gene JOURNAL Gene 41, 299-304 (1986) STANDARD full staff_review REFERENCE 3 (bases 11 to 241) AUTHORS Nishide,T., Emi,M., Nakamura,Y. and Matsubara,K. TITLE Corrected sequences of cDNAs for human salivary and pancreatic alpha-amylases JOURNAL Gene 50, 371-372 (1986) STANDARD full staff_review REFERENCE 4 (bases 1 to 251) AUTHORS Horii,A., Emi,M., Tomita,N., Nishide,T., Ogawa,M., Mori,T. and Matsubara,K. TITLE Primary structure of human pancreatic alpha-amylase gene: Its comparison with human salivary alpha-amylase gene JOURNAL Gene 60, 57-64 (1987) STANDARD full staff_entry COMMENT [Gene 50, 371-372 (1986)] revises [Gene 28, 263-270 (1984)]. [Gene 60, 57-64 (1987)] revises [Gene 28, 263-270 (1984)],[Gene 41, 299-304 (1986)],[Gene 50, 371-372 (1986)]. Draft entry and computer readable copy of sequence [Gene 28, 263-270 (1984)] kindly kindly provided by A.Horii (14-MAR-1988). [Gene 28, 263-270 (1984)] also sequenced the pancreatic alpha-amylase mRNA. Comparison of the two alpha-amylase nucleotide sequences reveals 96% homology in the coding region. Comparison of the sequences of human alpha-amylase cDNAs with those for mouse suggests the possibility of gene conversion between the two genes of alpha-amylase. FEATURES Location/Qualifiers intron <1..10 /note="alpha-amylase, intron C" exon 11..241 /note="alpha-amylase, exon 4 /map='1p21' /hgml_locus_uid='LJ0263N'" intron 242..>251 /note="alpha-amylase, intron D" BASE COUNT 69 a 50 c 60 g 72 t ORIGIN About 600 bp downstream of segment 3; chromosome 1p21. 1 acctcaacag gtcagagatt gtcgtctgtc tggtcttctc gatcttgcac tggggaagga 61 ttatgtgcgt tctaagattg ccgaatatat gaaccatctc attgacattg gtgttgcagg 121 gttcagaatt gatgcttcca agcacatgtg gcctggagac ataaaggcaa ttttggacaa 181 actgcataat ctaaacagta actggttccc ggaaggtagt aaacctttca tttaccagga 241 ggtacgtcaa t //