Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!uwm.edu!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Human pancreatic alpha-amylase (amy2) DNA, exon 1. Message-ID: Date: 8 May 91 12:01:55 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 76 Approved: lear@genbank.bio.net Checksum: 63597 5 LOCUS HUMAMY201 920 bp ds-DNA PRI 08-MAY-1991 DEFINITION Human pancreatic alpha-amylase (amy2) DNA, exon 1. ACCESSION M18714 K02213 KEYWORDS alpha-amylase; amylase. SEGMENT 1 of 10 SOURCE Human pancreas, cDNA to mRNA, clones pHPA76 and pHPA86 [Gene 28, 263-270 (1984)],[Gene 41, 299-304 (1986)],[Gene 50, 371-372 (1986)] and Human cosmid library DNA, clone cosP2 [Gene 60, 57-64 (1987)]. ORGANISM Homo sapiens Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae. REFERENCE 1 (bases 543 to 910) AUTHORS Nakamura,Y., Ogawa,M., Nishide,T., Emi,M., Kosaki,G., Himeno,S. and Matsubara,K. TITLE Sequences of cDNAs for human salivary and pancreatic alpha- amylases JOURNAL Gene 28, 263-270 (1984) STANDARD full staff_review REFERENCE 2 (bases 1 to 920) AUTHORS Nishide,T., Nakamura,Y., Emi,M., Yamamoto,T., Ogawa,M., Takesada,M. and Matsubara,K. TITLE Primary structure of human salivary alpha-amylase gene JOURNAL Gene 41, 299-304 (1986) STANDARD full staff_review REFERENCE 3 (bases 543 to 910) AUTHORS Nishide,T., Emi,M., Nakamura,Y. and Matsubara,K. TITLE Corrected sequences of cDNAs for human salivary and pancreatic alpha-amylases JOURNAL Gene 50, 371-372 (1986) STANDARD full staff_review REFERENCE 4 (bases 1 to 920) AUTHORS Horii,A., Emi,M., Tomita,N., Nishide,T., Ogawa,M., Mori,T. and Matsubara,K. TITLE Primary structure of human pancreatic alpha-amylase gene: Its comparison with human salivary alpha-amylase gene JOURNAL Gene 60, 57-64 (1987) STANDARD full staff_entry COMMENT [Gene 50, 371-372 (1986)] revises [Gene 28, 263-270 (1984)]. [Gene 60, 57-64 (1987)] revises [Gene 28, 263-270 (1984)],[Gene 41, 299-304 (1986)],[Gene 50, 371-372 (1986)]. Draft entry and computer readable copy of sequence [Gene 28, 263-270 (1984)] kindly provided by A.Horii (14-MAR-1988). [Gene 28, 263-270 (1984)] also sequenced the salivary alpha-amylase mRNA. Comparison of the two alpha-amylase nucleotide sequences reveals 96% homology in the coding region. Comparison of the sequences of human alpha-amylase cDNAs with those for mouse suggest the possibility of gene conversion between the two genes of alpha-amylase. FEATURES Location/Qualifiers exon 743..910 /note="alpha-amylase, exon 1 /nomgen='AMY2' /map='1p21'" intron 911..>920 /note="alpha-amylase, intron A" BASE COUNT 238 a 161 c 206 g 315 t ORIGIN 194 bp upstream of XbaI site; chromosome 1p21. 1 gtattcaagg ttaactgtgc ccgttggtat ctgtacatat ctttgatttc agtctttact 61 acacgtggct tggtcacttc atggctaaaa acatgcttgt ggaagaccag tctggctcgg 121 tgagtctgtg cggccagcag tctctgatct gtacagggta ttaatgtgtc agggctgagt 181 gttctgggat ttgtctagag gctggtaagg gcttctggac cacttgtttc tgtcctgtca 241 gtctgtcagg gttggaaagt ccaagccata ggacccagtt tcctttctta gcttacgtta 301 tctaccagag caccgtgggc tgttacttac cttgagttgg aaggggttcg catttatacc 361 tgtaaaagta ttcatccttt taatttatgt aaagtttttt tgtatgccat tctggatctt 421 taaagagatg acaacaaatt ttggttttct actgttatgt gagaacatta ggccccagca 481 acaggtcact gtttaaggaa aaataaaagt gctgccagaa cctaagaaaa acattaatat 541 ctaaaaggtc atttagatga tttccatgag agactttttg atgttcttta cctgttagga 601 ttattattga taatcctttt cagattatga ataaacagtt tgccctcaag tatttattca 661 tgctaatatt tactttgtaa aatgtgcttc ttacaggaat ataaatagtt tctggaaagg 721 acactgacaa cttcaaagca aaatgaagtt ctttctgttg cttttcacca ttgggttctg 781 ctgggctcag tattccccaa atacacaaca aggacggaca tctattgttc atctgtttga 841 atggcgatgg gttgatattg ctcttgaatg tgagcgatat ttagctccga agggatttgg 901 aggggttcag gtgggtatga //