Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!zaphod.mps.ohio-state.edu!wuarchive!uunet!stanford.edu!snorkelwacker.mit.edu!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Sea urchin (S.purpuratus) histone H1-H4 spacer inverted repeat. Message-ID: Date: 9 May 91 12:01:47 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 36 Approved: lear@genbank.bio.net Checksum: 19609 2 LOCUS SUSHISHA14 397 bp ds-DNA INV 09-MAY-1991 DEFINITION Sea urchin (S.purpuratus) histone H1-H4 spacer inverted repeat. ACCESSION K01794 KEYWORDS repetitive sequence. SOURCE Sea urchin (S.purpuratus; 7N) sperm DNA, clone 7N32L. ORGANISM Strongylocentrotus purpuratus Eukaryota; Animalia; Eumetazoa; Echinodermata; Echinozoa; Echinoidea; Echinacea; Echinoida; Strongylocentrotidae. REFERENCE 1 (bases 1 to 397) AUTHORS Vitelli,L. and Weinberg,E.S. TITLE An inverted sea urchin histone gene sequence with breakpoints between TATA boxes and mRNA cap sites JOURNAL Nucleic Acids Res. 11, 2135-2153 (1983) STANDARD simple staff_review COMMENT [Nucleic Acids Res. 11, 2135-2153 (1983)] reports a sea urchin histone gene fragment containing inverted regions of the normal repeat. Analysis of this fragment indicates that the H1-H4 spacer of one repeat is situated alongside the inverted H2A-H1 spacer of another repeat. The breakpoints in the original duplexes were found to be witin 4-6 bp of the H4 mRNA capsite and 8-10 bp of the H1 mRNA capsite in the standard repeat. FEATURES Location/Qualifiers repeat_region 1..247 /note="inverted repeat a" repeat_region 248..397 /note="inverted repeat b" BASE COUNT 93 a 113 c 102 g 89 t ORIGIN 1 bp upstream of XhoI site. 1 ctcgagcatt caacacaggc ctctctacat aaatgcatgt aataatgcta gcgaatactc 61 gccacgaggg ggcgcactcg aatggggagt ctccgcactc cagtcccgca taccgaatga 121 tgccgaatct cgtcacccaa gtccgcaatg gtgtaacaat actcgctgca atccggttga 181 ggctcggcaa cgccatcaat ataaggcatt gttgcgtacg tgggaggaga cagtcccggg 241 tcgtccgcgg acgacccggg actgtctcct cccacgtacg caacaatgcc ttatattgat 301 tgcggagact ccccattcga gtgcgccccc tcgtggcgag tattcgctag cattattaca 361 tgcatttatg tagagaggcc tgtgttgaat gctcgag //