Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!usc!apple!bionet!SPLAVA.CC.PLATTSBURGH.EDU!GRAZIAWD From: GRAZIAWD@SPLAVA.CC.PLATTSBURGH.EDU ("William D. Graziadei") Newsgroups: bionet.molbio.methds-reagnts Subject: Re: PUC 13 & RE Sites in Polylinker Message-ID: Date: 10 May 91 03:04:00 GMT Sender: daemon@genbank.bio.net Lines: 33 Date sent: 9-MAY-1991 21:57:09 Erica Cabot, asked..... > Someone in our lab has received a pUC13 clone and would like >to restriction map the insert. Specifically we'd like to >know which enzymes cut in the polylinker and which enzymes >don't cut in the vector at all. > > The problem is that none of the catalogs that we have on hand >list either pUC13 or m13mp13. > > > Does anyone out there know a supplier of either pUC13 or m13mp13? >Alternatively does anyone know the answer to the question I pose >in paragraph #1? Check in your library for "From Genes to Clones" by Ernst Winnacker, ISBN 0-89573-614-4 (see page 292). The following restriction enzymes cut in the polylinker of PUC 13: HindIII, PstI, (SalI, AccI, HincII), XbaI, BamHI, (XmaI, SmaI), SstI, EcoRI, and HaeIII. Hope this helps. Regards, Bill Send EMail replies to Bitnet or Internet address to by-pass the list. +-------------------------------------------------------------------------+ | Voice: (518) 846-7144 | William D. Graziadei, PhD | | Fax: (518) 846-7144 Ext 40 | In Vitro Cell Biology & | | (518) 564-7827 | Biotechnology | | Bitnet: GRAZIAWD@SNYPLAVA | SUNY Plattsburgh/Miner Inst| |Internet: GRAZIAWD@SPLAVA.CC.PLATTSBURGH.EDU| Chazy, New York 12921 | +-------------------------------------------------------------------------+