Path: utzoo!utgpu!news-server.csri.toronto.edu!bonnie.concordia.ca!nstn.ns.ca!uupsi!fernwood!apple!olivea!samsung!usc!rutgers!tut.cis.ohio-state.edu!ucunix.san.uc.edu!zuazaga From: zuazaga@ucunix.san.uc.edu (Humberto Oritz-Zuazaga) Newsgroups: bionet.neuroscience Subject: Substraction Libraries? Message-ID: <1991May11.195129.26462@ucunix.san.uc.edu> Date: 11 May 91 19:51:29 GMT Sender: zuazaga@ucunix.san.uc.edu (Humberto Oritz-Zuazaga) Organization: Univ. of Cincinnati Lines: 19 I am posting this note on behalf of my wife, who reads this group over my shoulder. Replies to me or the net, etc. I am thinking about determining what neuronal genes are induced following excitation, besides the immediate-early genes. I have been told that substraction libraries may be of help but frankly I have no idea of what they entail and how this procedure will help me. If anyone has any experience with substraction libraries I would appreciate information on their applications and how they are actually done. Sandra Pena Molecular Neurotoxicology Program Dept. of Environmental Health University of Cincinnati -- Humberto Ortiz-Zuazaga University of Cincinnati Physiology & Biophysics zuazaga@ucunix.san.uc.edu Cincinnati OH 45267-0576