Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!zaphod.mps.ohio-state.edu!caen!news.cs.indiana.edu!bionet!NRCCAD.NRC.CA!NUM208JN From: NUM208JN@NRCCAD.NRC.CA (John Nash) Newsgroups: bionet.software Subject: re:pUC vectors Message-ID: <7716B487D95F001F44@NRCNET.NRC.CA> Date: 14 May 91 01:26:00 GMT Sender: daemon@genbank.bio.net Lines: 58 G'day folk, I saw this today... *Date: Mon, 13 May 91 18:34 CDT *From: Bruce Roe *Subject: re: pUC vectors *To: bio-soft@genbank.bio.net Shouldn't this be "methods @genbank.bio.net"? Not a complaint from me, just that it may get a more aappropriate audience there. *X-Vms-To: IN%"bio-soft@genbank.bio.net" *You wrote: *>> Hi there, *>> we are looking for a simple pUC vector (preferably pUC 18 *>> or 19) with a T7 promoter and a T3 promoter cloned in the *>> polylinker. *>> We already have pUC118, but this vector contains, as you know, *>> an origin for single stranded replication, and this hampers *>> our cloning scheme. Shortened for brevity... and *>But what we really want is a vector that looks like this: pUC sequences- *>T7 promoter-polylinker-T3 promoter-pUC sequences. Simple ? Yes ! *>M13 ori for ss replication ? No ! and * Bethesda Research Labs. has to vectors called: *pT7/T3a-18 and pT7/T3a-19. or at least they do according to their *catalogue. They both are pUC bases and have either the pUC18 or 19 *multiple cloning site flanked by the T7 and 73 promoters. I use the pT7/T3-alpha vectors, and I hate to report that they have M13 (or f1) ss ori sites. Sorry to be a bubble burster, but I think that Clemens may have to start constructing ;-( cheers, John, -------------------------------------------------------------- John H.E. Nash Institute for Biological Sciences, National Research Council of Canada, Ottawa, Canada K1A 0R6. Phone: (613) 990-0990 Fax: (613) 952-9092. ============================================================== P.S. It's a BOY!