Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!zaphod.mps.ohio-state.edu!caen!news.cs.indiana.edu!bionet!AARDVARK.UCS.UOKNOR.EDU!BROE
From: BROE@AARDVARK.UCS.UOKNOR.EDU (Bruce Roe)
Newsgroups: bionet.software
Subject: re: pUC vectors
Message-ID: <9105132335.AA22870@genbank.bio.net>
Date: 13 May 91 23:34:00 GMT
Sender: daemon@genbank.bio.net
Lines: 44

You wrote:

>>  Hi there,
>>  we are looking for a simple pUC vector (preferably pUC 18
>>  or 19) with a T7 promoter and a T3 promoter cloned in the
>>  polylinker. 
>>  We already have pUC118, but this vector contains, as you know, 
>>  an origin for single stranded replication, and this hampers
>>  our cloning scheme. 

>>  If you have this kind of vector, we would be very pleased to hear
>>  from you.

>>  Christian and Clemens

>this message is apparently very confusing, since we were recieved many
>answers (for which we are grateful) that we should use bluescript (or
>could it be that only few people know that this vector contains an ori
>for single stranded replication ?).
>But what we really want is a vector that looks like this: pUC sequences-
>T7 promoter-polylinker-T3 promoter-pUC sequences. Simple ? Yes !
>M13 ori for ss replication ? No !

>Before I start constructing myself, I would like to hear from anybody out
>there, whether he has this rather simple construct, or one like it.

>Clemens

	Bethesda Research Labs. has to vectors called:
pT7/T3a-18 and pT7/T3a-19.  or at least they do according to their
catalogue.  They both are pUC bases and have either the pUC18 or 19
multiple cloning site flanked by the T7 and 73 promoters.  

	Cheers,

 ******************************  Bruce A. Roe  ******************************
 *  Professor of Chemistry and Biochemistry     Dept. of Chem. and Biochem. *
 *  INTERNET: BROE@aardvark.ucs.uoknor.edu      University of Oklahoma      *
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 *  FAXnet:   405-325-6111                                                  *
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