Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!zaphod.mps.ohio-state.edu!samsung!news.cs.indiana.edu!bionet!mcoiarc.bitnet!TAO%OPUS From: TAO%OPUS@mcoiarc.bitnet Newsgroups: bionet.software Subject: Re: pUC vectors Message-ID: <770CA5A243DF0056BC@MCOIARC.BITNET> Date: 14 May 91 02:38:00 GMT Sender: daemon@genbank.bio.net Lines: 31 I could not help giving my 2 cents worth regarding the posting on pUC vecter. It seems to me that people of the electronic age are even more narrowly-minded than their "ancestors". Maybe they are lazier too. Answer to the question is the pBS vector from Strategene. Even if one doesn't have the money to buy it, one could still do some simple restriction digestion and ligation to satisfy the experimental need [since the pUC based vector is available to the questioneer, with an unwanted feature, right?]. Problems tend to be unique for individual project, customerization usually would be the only way out. To be a little deligent and broad-minded will always help. Another thing is whoever answering the posting is actually doing the questioneer a favor. I hope that everyone could keep this in mind. Tao Tao "My name has nothing to do with the Graduate Student Taoism, even though I am a believer of it." Department of Microbiology Medical College of Ohio Toledo, Ohio 43699-0008