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From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E. coli left end of transposon Tn7 inserted into glmS terminator
Message-ID: <May.18.05.09.46.1991.25307@genbank.bio.net>
Date: 18 May 91 12:09:47 GMT
Article-I.D.: genbank.May.18.05.09.46.1991.25307
Sender: root@genbank.bio.net
Distribution: bionet
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Approved: lear@genbank.bio.net
Checksum: 54377     4

LOCUS       ECOTN7GLM     574 bp ds-DNA             BCT       18-MAY-1991
DEFINITION  E. coli left end of transposon Tn7 inserted into glmS terminator
ACCESSION   X03474 M24254
KEYWORDS    glmS gene; glutamine amidotransferase; terminator; transferase;
            transposon; unidentified reading frame.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
            Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Gay,N.J., Tybulewicz,V.L. and Walker,J.E.
  TITLE     Insertion of transposon Tn7 into the Escherichia coli glmS
            transcriptional terminator
  JOURNAL   Biochem. J. 234, 111-117 (1986)
  STANDARD  full automatic
COMMENT     SWISS-PROT; P13988; TNSA$ECOLI.
            
            *source: strain=GD92::Tn7. see also V00620 Transposition of Tn7
            into E. coli glmS transcriptional terminator abolishes
            transcription termination at this site; the transcript now extends
            into the left end of Tn7 and terminates at a new site "tm", 127
            base pairs from the left end of Tn7. This region of the transposon
            contains a long open reading frame which encodes a protein sequence
            that is significantly related to the transposase proteins of the
            transposable elements IS1 and Tn3.
            
            From EMBL    entry ECTN7GLM;  dated 21-FEB-1991.
FEATURES             Location/Qualifiers
     CDS             <1..9
                     /product="glutamine amidotransferase"
                     /gene="glmS"
                     /codon_start=1
     misc_feature    17..37
                     /note="transcription terminator fragment (glmS)"
     repeat_unit     17..28
                     /note="imp. inverted repeat A"
     repeat_unit     33..37
                     /note="interrupted imp. inverted repeat A'"
     transposon      38..>574
                     /note="inserted Tn7"
     promoter        117..123
                     /note="put. -35 region (Tn7)"
     promoter        140..145
                     /note="put. -10 region (Tn7)"
     misc_feature    148..148
                     /note="put. transcription initiation site (Tn7)"
     misc_signal     164..164
                     /note="put. new transcription termination site, tm site
                     (glmS)"
     CDS             172..>574
                     /note="Tn7 URF1"
                     /codon_start=172
BASE COUNT      165 a    105 c    133 g    171 t
ORIGIN
        1 acggttgagt aataaatgga tgccctgcgt aagcgggtgt gggcggacaa taaagtctta
       61 aactgaacaa aatagatcta aactatgaca ataaagtctt aaactagaca gaatagttgt
      121 aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt tatggctaaa
      181 gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg gcgtggccaa
      241 gggcatggta aagactatat tccatggcta acagtacaag aagttccttc ttcaggtcgt
      301 tcccaccgta tttattctca taagacggga cgagtccatc atttgctatc tgacttagag
      361 cttgctgttt ttctcagtct tgagtgggag agcagcgtgc tagatatacg cgagcagttc
      421 cccttattac ctagtgatac caggcagatt gcaatagata gtggtattaa gcatcctgtt
      481 attcgtggtg tagatcaggt tatgtctact gattttttag tggactgcaa agatggtcct
      541 tttgagcagt ttgctattca agtcaaacct gcag
//