Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!zaphod.mps.ohio-state.edu!wuarchive!uunet!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Vicia faba mRNA for Legumin A1 pre-pro-polypeptide Message-ID: Date: 22 May 91 12:19:37 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 123 Approved: lear@genbank.bio.net Checksum: 06242 7 LOCUS VFALEA2 1666 bp ss-mRNA PLN 22-MAY-1991 DEFINITION Vicia faba mRNA for Legumin A1 pre-pro-polypeptide ACCESSION X55014 KEYWORDS legumin A; storage protein. SOURCE Vicia faba RNA. ORGANISM Vicia faba Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida; Rosidae; Rosales; Fabaceaea. REFERENCE 1 (sites) AUTHORS Bassuener,R., Wobus,U. and Rapoport,T.A. TITLE Signal recognition particle triggers the translocation of storage globulin polypeptides from field beans (Vicia faba L.) across mammalian endoplasmic reticulum membrane. JOURNAL FEBS Lett. 166, 314-320 (1984) STANDARD full staff_review REFERENCE 2 (sites) AUTHORS Horstmann,C. TITLE New aspects of the subunit structure of Vicia faba legumin. JOURNAL Kulturpflanze 32, 109-116 (1984) STANDARD full staff_review REFERENCE 3 (bases 1 to 1666) AUTHORS Bassuener,R. JOURNAL Unpublished (1990) STANDARD full automatic REFERENCE 4 (bases 1 to 1666) AUTHORS Schlesier,B., Bassuener,R., Nong,V.H. and Muentz,K. TITLE The cDNA derived primary structure of two distinct legumin A subunit precursors from field bean (Vicia faba L.) JOURNAL Nucleic Acids Res. 18, 7146-7146 (1990) STANDARD full staff_review COMMENT *source: strain=var. minor cv.Fribo, clone= Vfle 129 author's comment:"The supposed site of polyadenylation is four bp downstream of the reported 3' terminal nucleotide." "For legumin A encoding cDNA's of Vicia faba see: Wobus et al.: FEBS Lett. 201: 74-80(1986) and X55013; for legumin B encoding genes of Vicia faba see: X03677; X14237; X14238; X14239; X14240; X14241." "The classification is according to Nielsen et al.: The Plant Cell 1: 313-328(1989), as compared to other legumin-like seed storage globulin subunits." From EMBL 26 entry VFLEA2; dated 05-APR-1991. FEATURES Location/Qualifiers mRNA <1..>1666 /partial /gene="LeA2" /citation=[4] /citation=[1] sig_peptide 24..86 /product="signal peptide for cotranslational translocation" CDS 24..1526 /product="legumin A2 primary translation product" /gene="LeA2" /codon_start=24 /citation=[4] /citation=[1] /citation=[2] mat_peptide 87..968 /note="The legumin mature subunit consists of one acidic alpha and one basic beta chain, linked by a disulfide bridge, and generated from the described pro-polypeptide precursor." /product="legumin A2 acidic alpha chain" /gene="LeA2" /citation=[4] /citation=[2] CDS 87..1526 /product="legumin A2 pro-polypeptide" /gene="LeA2" /codon_start=87 /citation=[4] /citation=[1] misc_feature 357..395 /note="hydrophilic segment" misc_feature 576..644 /note="hydrophilic segment" misc_feature 801..965 /note="hydrophilic segment" repeat_region 840..857 /rpt_type=tandem /rpt_unit=840..845 mat_peptide 969..1523 /note="The mature legumin subunit consists of one acidic alpha and one basic beta chain, linked by a disulfide bridge, and generated from the described pro-polypeptide precursor." /product="legumin A2 basic beta chain" /gene="LeA2" /citation=[4] /citation=[2] polyA_signal 1643..1648 BASE COUNT 537 a 359 c 385 g 385 t ORIGIN 1 acattctcta aatatctgtt atcatggcta aacttcttgc actctctctt tcactttgct 61 ttctactttt cagtagctgc tttgctctca gagaacagtc tcaacaaaat gagtgtcagc 121 tagaacgcct cgatgccctt gaaccagata accgtataga gtcagaaggt ggacttattg 181 agacttggaa tccaaacaac aggcaattcc gatgtgcaag cgtggccctc tctcgtgcta 241 cccttcagcg caatgccctt cgcagacctt actattccaa tgctccccaa gaaatttaca 301 tccaacaagg taatggatat tttggcatgg tattccctag ttgtcctgag acctttgagg 361 agccccaaca atctgaacaa ggagagggag gcaggtacag agacagccac caaaaggtta 421 accgatttag agagggtgat atcattgcag ttcctactgg tattgtattt tggatgtaca 481 atgaccaaga tactccagtt attgccattt ctcttactga tattggcagc tccaataacc 541 agcttgatca gatgcctagg agattctatc ttgctgggaa ccaagagcaa gagtttctac 601 gataccaaca tcaacaagga ggaaaggaag aacaagacaa tgacgggaac aacattttca 661 gcggcttcaa gagggatttc ttggaagatg cgttgaacgt gaacaggcat atagtagaca 721 gacttcaagg caggaacgaa gacgaagaga agggagccat tgtcaaagtg aaaggtggtc 781 tcagcatcat aacccctccg gagaggcaag cgcgccaccc gagaggcagc agacaagaag 841 aagatgaaga tgaagatgaa aaggaagaga gacaaccaag ccatcataaa agcagaagag 901 gtgaagatga agacgacaag gaaaagcgcc acagccaaaa aggtgaaagc agaaggcatg 961 gagacaatgg gcttgaggaa accgtttgca ctgccaaact tcggctgaac attggttcat 1021 cttcatcgcc agacatctac aaccctcaag ctggtagaat caaaactgtt accagccttg 1081 acctcccagt tctcaggtgg ctcaaactaa gtgctgagca tggatctctc cgtaaaaatg 1141 ctatgtttgt gcctcactac aacctcaacg caaacagcat attatacgca ttgaagggac 1201 gtgcaaggct acaagttgtg aactgcaatg gcaacactgt gtttgatgaa gagctagaag 1261 ccggtcgtgc attgacagtg ccacaaaact atgctgtggc tgcaaaatca ttgagcgaca 1321 ggttcacata tgtagcattc aagaccaatg atagagctgg tattgcaaga cttgcaggga 1381 catcatcagt tataaatgat atgccggtgg atgtggttgc agctacattc aacttggaga 1441 ggaatgaggc aaggcagctc aagtccaaca atcccttcaa atttctagtt ccaccgcgtg 1501 agtctcagaa gagagcttcc gcttagattt gtcatcaaat cgatgaaaat aataatgaaa 1561 ataatgaata agaaaactaa ggcttagatg cctatgttgt tggtgtaaaa taacacgagt 1621 catgtacctt ttgcggcaac agaataaaca aagaagcgaa gttcca //