Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!zaphod.mps.ohio-state.edu!wuarchive!uunet!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Vicia faba mRNA for Legumin A1 pre-pro-polypeptide Message-ID: Date: 22 May 91 12:19:36 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 127 Approved: lear@genbank.bio.net Checksum: 19941 7 LOCUS VFALEA1 1585 bp ss-mRNA PLN 22-MAY-1991 DEFINITION Vicia faba mRNA for Legumin A1 pre-pro-polypeptide ACCESSION X55013 KEYWORDS legumin A; storage protein. SOURCE Vicia faba RNA. ORGANISM Vicia faba Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida; Rosidae; Rosales; Fabaceaea. REFERENCE 1 (sites) AUTHORS Bassuener,R., Wobus,U. and Rapoport,T.A. TITLE Signal recognition particle triggers the translocation of storage globulin polypeptides from field beans (Vicia faba L.) across mammalian endoplasmic reticulum membrane. JOURNAL FEBS Lett. 166, 314-320 (1984) STANDARD full staff_review REFERENCE 2 (sites) AUTHORS Horstmann,C. TITLE New aspects of the subunit structure of Vicia faba legumin. JOURNAL Kulturpflanze 32, 109-116 (1984) STANDARD full staff_review REFERENCE 3 (bases 1 to 1585) AUTHORS Bassuener,R. JOURNAL Unpublished (1990) STANDARD full automatic REFERENCE 4 (bases 1 to 1585) AUTHORS Schlesier,B., Bassuener,R., Nong,V.H. and Muentz,K. TITLE The cDNA derived primary structure of two distinct legumin A subunit precursors from field bean (Vicia faba L.) JOURNAL Nucleic Acids Res. 18, 7146-7146 (1990) STANDARD full staff_review COMMENT *source: strain=var. minor cv.Fribo clone=Vfle 165 Author's comment:For legumin A encoding cDNA's of Vicia faba see: Wobus et al.: FEBS Lett. 201:74-80(1986) and x55014. For legumin B encoding genes of Vicia faba see: X03677; X14237; X14238;X14239; X14240; X14241." "The classification is according to Nielsen et al.: The Plant Cell 1:313-328 (1989), as compared to other legumin-like storage globulin subunits." From EMBL 26 entry VFLEA1; dated 05-APR-1991. FEATURES Location/Qualifiers mRNA <1..>1585 /partial /gene="LeA1" /citation=[4] /citation=[1] mat_peptide <1..926 /codon_start=3 /note="The region encoding nine amino acids following the N terminus of this chain are not present in the reported cDNA." /product="legumin A1 acidic alpha chain (N-terminal incomplete)" /gene="LeA1" /citation=[4] /citation=[2] CDS <1..1496 /partial /codon_start=3 /note="The region encoding the signal peptide and following nine amino acids are not present in the reported cDNA." /product="N-terminal incomplete legumin A1 pre-pro-polypeptide" /gene="LeA1" /codon_start=1 /citation=[4] /citation=[1] /citation=[2] misc_feature 246..284 /note="hydrophilic segment" misc_feature 465..602 /note="see: Nielsen et al.: The Plant Cell 1:313-328(1989). For legumin A1 this variable region is organized as a 69bp tandem repeat: from 465 to 633, and from 634 to 602, never described before." misc_feature 753..923 /note="hydrophilic segment" repeat_region 792..815 /rpt_type=tandem /rpt_unit=792..797 mat_peptide 927..1493 /note="The legumin mature subunit consists of one acidic alpha and one basic beta chain linked by a disulfide bridge, and generated from the described pro-polypeptide precursor." /product="legumin A1 basic beta chain" /gene="LeA1" /citation=[4] /citation=[2] repeat_region 1437..1498 /rpt_type=tandem /rpt_unit=1437..1467 /note="This 31bp tandem repeat unit is described for the first time. It is unusual as compared to other repetetive structures in legumin cDNA since it causes a shift in the reading frame (see legumin A2)." BASE COUNT 522 a 337 c 379 g 347 t ORIGIN 1 agtgtcagct agaacgcctc gatgcccttg aaccagataa ccgtatagag tcagaaggtg 61 gactcattga gacttggaat ccaaacaaca ggcaattccg atgtgcaaga gtggcactct 121 ctcgtgctac ccttcaacgc aatgcccttc gcagacctta ctattccaat gctccccaag 181 aaatttacat ccaacaaggt aatggatatt ttggcatggt attccctagt tgtcctgaga 241 cctttgagga gccacaacaa tctgagcaag gagaaggacg caggtacaga gacagccacc 301 aaaaggttaa cagattcaga cagggtgata tcattgcagt tcctactggt attgtatttt 361 ggatgtacaa tgaccaagat attccagtta ttgccatttc tcttactgat actggcagct 421 ccaataacca gcttgatcag atgcctagga gattctatct tgctgggaat caagagcaag 481 agtttctacg ataccaacat caacaaggag taaaggaaga acaagacaat gacgggaacc 541 aagagcaaga gtttctacga taccaacatc gacaaggagt aaaggaagaa caagacaatg 601 acgggaacaa cattttcagc ggcttcaaca gggatttctt ggaagatgct ttcaacgtga 661 acaggcatat agtagacaga cttcaaggca ggaacgaaga gaggggagcc attgtcaaag 721 tgaaaggtgg tctcagcatc ataacccctc cggagaggca agcgcgccac ccgagaggca 781 gcagacaaga agaagatgaa gatgaagatg aagatgaaaa ggaagagaga caaccaagcc 841 atcataaaag cagaagaggt gaagatgaag acgacaagga aaagcgccac agccaaaaag 901 gtgaaagcag aaggcatgga gacaatgggc ttgaggaaac cgtttgcact gccaaacttc 961 ggctgaacat tggttcatct tcatcgccag acatctacaa ccctcaagct ggtagaatca 1021 aaactgttac cagcgttgac ctcccagttc tcaggtggct caaactaagt gctgagcatg 1081 gatctctccg caaaaatgct atgtttgtgc ctcactacaa cctcaacgca aacagcgtat 1141 tatacgcatt gaagggacgt gcaaggctac aagttgtgaa ctgcaatggc aacactgtgt 1201 ttgatggaga gctagaagcc ggtcgtgcat tgacagtgcc acaaaactat gttgtggctg 1261 caaaatcatt gagcgacagg ttcacatatg tagcattcaa gacaaatgac agagctggta 1321 ttgcaagact agcagggaca tcatcagtta taaatgatct gcctttggat gtagtcgcag 1381 ctacattcaa cttggagagg aatgaggcaa ggcagctcaa gttcaacaat ccttccagat 1441 ttctagttcc accacgcgag tctgagaaga tttctagttc caccacgcga gtatgagaag 1501 agagcttccg cttagatttg tcatcaaatc gatgaaaata ttaataatga ataagaaagg 1561 taaggcttaa atggctttgt tgttg //