Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!think.com!spool.mu.edu!munnari.oz.au!ariel!ucsvc.ucs.unimelb.edu.au!luga!lure.latrobe.edu.au!micprf
From: micprf@lure.latrobe.edu.au
Newsgroups: bionet.molbio.methds-reagnts
Subject: Electroporation efficiency in E. coli
Message-ID: <1991May23.181415.1@lure.latrobe.edu.au>
Date: 23 May 91 08:14:15 GMT
Article-I.D.: lure.1991May23.181415.1
Sender: news@luga.latrobe.edu.au (USENET News System)
Organization: VAX Cluster, Computer Centre, La Trobe University
Lines: 10

Greetings.
We recently bought an electroporator and have been doing some experiments
to optimize it for our standard E. coli host strains. At present we are
getting about 2 x 10^7 per microgram for HB101 and 10 times that with
MC1061. These numbers apply to two different vectors - a 6.1 kB Dictyostelium
shuttle vector and the Bluescript vector. These frequencies are well short
of the frequencies one is supposed to be able to achieve. My question is -
what are the real frequencies that most people achieve with cells they have
prepared themselves?			Cheers, Paul Fisher.
						(micprf@lure.latrobe.edu.au)