Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!usc!ucla-cs!usenet From: edu%aids@cs.ucla.eduSupport Account for SCI.MED.AIDS 23-MAY-19 Newsgroups: sci.med.aids Subject: Re: (3178) Re: viral amplification Message-ID: <1991May24.022033.11956@cs.ucla.edu> Date: 23 May 91 19:20:00 GMT Sender: usenet@cs.ucla.edu (Mr. News Himself) Organization: UCLA, Computer Science Department Lines: 64 Approved: ddodell@stjhmc.fidonet.org (David Dodell) Note: non-commercial reproduction. Nntp-Posting-Host: squid.cs.ucla.edu Archive-Number: 3180 91 11:57:39.98 To: N M CC: Subj: (3178) Re: viral amplification Received: From USCVM(MAILER) by FORDMULC with Jnet id 1034 for MADLANSACAY@FORDMULC; Thu, 23 May 91 11:57 EST Received: by USCVM (Mailer R2.08) id 2124; Thu, 23 May 91 08:58:34 PDT Date: Thu, 23 May 91 09:00:46 pdt Reply-To: "Sci.Med.AIDS Newsgroup" Sender: "Sci.Med.AIDS Newsgroup" From: "Support Account for SCI.MED.AIDS" Subject: (3178) Re: viral amplification X-To: AIDS%RUTVM1.BITNET@CUNYVM.CUNY.EDU To: N M >From: bwoodman@magnus.acs.ohio-state.edu (Robert H Woodman) Newsgroups: sci.med.aids Approved: phil@wubios.wustl.edu Subject: Re: viral amplification References: <1991May23.135630.5906@cs.ucla.edu> Sender: news@magnus.acs.ohio-state.edu Date: Thu, 23 May 1991 15:35:12 GMT Distribution: world Organization: The Ohio State University Keywords: antibodies, PCR Note: Copyright 1990 by Daniel R. Greening. Permission granted for Note: non-commercial reproduction. Archive-number: 3178 In article <1991May23.135630.5906@cs.ucla.edu> writes: >I have read in one of messages in this list that one can be >HIV antibody negative (through ELISA) and be viral positive (through >cell viral amplification). Can anyone send me more info on this. >Where can you get that cell amplification tests? Are there research done >for more advanced antibody testing? > >Thanks The amplification test you refer to is based on a new molecular biology tech- nique called PCR (short for 'Polymerase Chain Reaction'). Whereas the ELISA test, looks only for the presence of HIV antibodies (which means that you have been infected and that your body has responded by recognizing the protein coat of the virus and producing antibodies to that coat), PCR looks for actual RNA or DNA (if the virus has reverse transcribed and integrated into the chromo- somes of your cells) sequences of HIV. This technique allows detection of even single viral copies in cells, and if done correctly (much easier said than done) it can allow quantitation of the virus load in the patient's body. I do not know where you could go to get such a test, since I am in no way fa- miliar with the area of the country where you live, but any hospital or re- search facility engaged in HIV research should be able to fill you in on the test and where you can go to get it. Bob Woodman -- ********************************************************************* *Robert H. "Bob" Woodman, PhD * "A job not worth doing well is not * *INTERNET: woodman.1@osu.edu * worth doing."--Salvador Luria * ********************************************************************* <^>v Via SCI.MED.AIDS => AIDSNEWS gateway / aids@cs.ucla.edu