Path: utzoo!utgpu!watserv1!watmath!att!emory!wuarchive!zaphod.mps.ohio-state.edu!think.com!linus!agate!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Human mRNA for immunoglobulin heavy chain VDJ region, Vh-IV family Message-ID: Date: 27 May 91 12:24:07 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 55 Approved: lear@genbank.bio.net Checksum: 61125 3 LOCUS HUMFOGBH 438 bp ss-mRNA PRI 27-MAY-1991 DEFINITION Human mRNA for immunoglobulin heavy chain VDJ region, Vh-IV family (cell line Fog-B) ACCESSION X52110 KEYWORDS immunoglobulin; immunoglobulin heavy chain; joining region; variable region. SOURCE Homo sapiens RNA. ORGANISM Homo sapiens Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae. REFERENCE 1 (bases 1 to 438) AUTHORS Hughes-Jones,N.C. JOURNAL Unpublished (1990) STANDARD full automatic REFERENCE 2 (bases 1 to 438) AUTHORS Hughes-Jones,N.C., Bye,J.M., Beale,D. and Coadwell,J. TITLE Nucleotide sequences and three-dimensional modelling of the Vh and Vl domains of two human monoclonal antibodies specific for the D antigen of the human Rh-blood-group system. JOURNAL Biochem. J. 268, 135-140 (1990) STANDARD full automatic COMMENT *source: strain=blood donor FO; cell type=B lymphocytes; *source: cell line=Fog-B; **map: chromosome=14; From EMBL entry HSFOGBH; dated 07-JUL-1990. FEATURES Location/Qualifiers CDS 1..>438 /note="precursor (AA -19 to 127)" /codon_start=1 CDS 1..57 /note="signal peptide (AA -19 to -1)" /codon_start=1 CDS 58..>438 /note="VDJ region (AA 1-127)" /codon_start=58 CDS 58..351 /note="Vh region (AA 1-98)" /codon_start=58 CDS 352..405 /note="D region (AA 99-116)" /codon_start=352 CDS 406..>438 /note="J6 region (AA 117-127)" /codon_start=406 BASE COUNT 83 a 126 c 131 g 98 t ORIGIN 1 atgaagcacc tgtggttctt cctcctgctg gtggcggctc ccagatgggt cctgtcccag 61 ctgcggctgc aggagtcggg cccaggactg gtgaagcctt cggagaccct gtccctcacc 121 tgcagtgtct ctggtggctc cgtcagcagt ggtggtctct actggggctg ggtccgccag 181 cccccaggga aggggctcga atggattggc agtatatttt atagtgggag cacctactac 241 aatccctccc tcaagagccg agtcaccata tccgtagaca cgttgaagaa taacttctcc 301 ctgaagctga gttctgtgac cgccgcagac acggctgttt attactgtac gagaccaggc 361 tatggcgaca cctcggtacg gaagagggtt tggaatatgg acctctgggg ccaagggacc 421 acggtcaccg tctcctcg //