Path: utzoo!utgpu!watserv1!watmath!att!news.cs.indiana.edu!sdd.hp.com!wuarchive!uunet!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Human germline gene for the leader peptide and variable region of a Message-ID: Date: 27 May 91 12:26:43 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 67 Approved: lear@genbank.bio.net Checksum: 41907 4 LOCUS HUMIGK3 1181 bp ds-DNA PRI 27-MAY-1991 DEFINITION Human germline gene for the leader peptide and variable region of a kappa immunoglobulin (subgroup V kappa I) ACCESSION Z00001 V00559 KEYWORDS germline; immunoglobulin; kappa-immunoglobulin; variable region. SOURCE Homo sapiens DNA. ORGANISM Homo sapiens Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae. REFERENCE 1 (bases 176 to 975) AUTHORS Bentley,D.L. and Rabbitts,T.H. TITLE Human immunoglobulin variable region genes - DNA sequences of two V kappa genes and a pseudogene JOURNAL Nature 288, 730-733 (1980) STANDARD full automatic REFERENCE 2 (bases 1 to 1181) AUTHORS Jaenichen,H.R. JOURNAL Unpublished (1984) STANDARD full automatic REFERENCE 3 (bases 1 to 1181) AUTHORS Jaenichen,H.R., Pech,M., Lindenmaier,W., Wildgruber,N. and Zachau,H.G. TITLE Composite human V kappa genes and a model of their evolution JOURNAL Nucleic Acids Res. 12, 5249-5263 (1984) STANDARD full automatic COMMENT The sequence of this entry is identical to the published sequence of HK102 (ref. [1] and entry HSIGK3), but it extends it in both directions; the sequence is designated V1 in refs. [2] and [3]. From EMBL entry HSIGK3; dated 03-JAN-1991. FEATURES Location/Qualifiers CDS join(297..351,477..772) /note="leader peptide" /codon_start=297 misc_feature 168..182 /note="put. regulatory element pd" misc_feature 200..209 /note="put. regulatory element dc" misc_feature 242..249 /note="TATA-box" intron 352..476 /note="intron I" misc_feature 775..781 /note="heptanucleotide box" BASE COUNT 318 a 296 c 259 g 308 t ORIGIN 1 ctgaggaata tttaaaattc tcaaaaaaat acctaaaaat tgtttctcat aataaaatag 61 tccccagtag aaacacattc tctgcagaca aatttgtgct accctggtct tacctgggac 121 acctggggac actgagctgg tgctgagtta ctgagatgag ccagctctgc agctgtgccc 181 agcctgcccc atcccctgct catttgcatg ttcccagagc acaacctcct gccctgaagc 241 cttattaata ggctggtcac actttgtgca ggagtcagac tcagtcagga cacagcatgg 301 acatgagggt ccccgctcag ctcctggggc tcctgctgct ctggctccca ggtaaggaag 361 gagaacacta ggaatttact cagcccagtg tgctcagtac tgcctggtta ttcagggaag 421 tcttcctata atatgatcaa tagtatgaat atttgtgttt ctatttccaa tctcaggtgc 481 caaatgtgac atccagatga cccagtctcc ttccaccctg tctgcatctg taggagacag 541 agtcaccatc acttgccggg ccagtcagag tattagtagc tggttggcct ggtatcagca 601 gaaaccaggg aaagccccta agctcctgat ctatgatgcc tccagtttgg aaagtggggt 661 cccatcaagg ttcagcggca gtggatctgg gacagaattc actctcacca tcagcagcct 721 gcagcctgat gattttgcaa cttattactg ccaacagtat aatagttatt ctcccacagt 781 gttacacacc cgaacataaa cccccaggga agcagatgtg tgaggcaggg ctgccccagc 841 tgctcctcct gatgcctcta tcagctgaga gtggtcctca gatgcagcca cactctgatg 901 gtgttggtag agggggacat ggagtcacct ctgcacccta attcttttct ctttctcagc 961 cccaactgca cagatctagc aatgcctctc ctgatttaat aaagacagag atcatgacac 1021 ctgaagagtc tagtttatgg cttcagctgg actttatata acagagaaga ggccactata 1081 gatattcaaa gcaggaattg tcttaataca gacaattaga gtctaaacta ctgaagtcta 1141 aataaaatgt agagatgaat ctctaaattt aatgttttac g //