Path: utzoo!utgpu!watserv1!watmath!att!news.cs.indiana.edu!sdd.hp.com!wuarchive!uunet!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Human germline pseudogene for the leader peptide and variable Message-ID: Date: 27 May 91 12:26:46 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 83 Approved: lear@genbank.bio.net Checksum: 10928 5 LOCUS HUMIGK6 1247 bp ds-DNA PRI 27-MAY-1991 DEFINITION Human germline pseudogene for the leader peptide and variable region of a kappa immunoglobulin (subgroup V kappa I) ACCESSION Z00004 X00748 KEYWORDS germline; immunoglobulin; kappa-immunoglobulin; pseudogene; variable region. SOURCE Homo sapiens DNA. ORGANISM Homo sapiens Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae. REFERENCE 1 (bases 1 to 1247) AUTHORS Jaenichen,H.R. JOURNAL Unpublished (1984) STANDARD full automatic REFERENCE 2 (bases 1 to 1247) AUTHORS Jaenichen,H.R., Pech,M., Lindenmaier,W., Wildgruber,N. and Zachau,H.G. TITLE Composite human V kappa genes and a model of their evolution JOURNAL Nucleic Acids Res. 12, 5249-5263 (1984) STANDARD full automatic COMMENT The sequence is designated V52 in refs. [1] and [2]; the variable region gene segment is inactivated by a frameshift mutation (insertion of a G) at the position 886) From EMBL entry HSIGK6; dated 06-JUL-1989. FEATURES Location/Qualifiers misc_feature 4..47 /note="simple sequence region" unsure 69..69 /note="A or G or T" unsure 77..77 /note="A or C or T" unsure 89..89 /note="A or C" unsure 211..211 /note="A or G or T" unsure 349..349 /note="A or T" misc_feature 416..425 /note="defective (due to a deletion) put. regulatory element pd" misc_feature 427..436 /note="put. regulatory element dc" promoter 470..477 /note="TATA-box" CDS 525..579 /note="first exon (leader peptide) (579 is 1st base in codon)" /codon_start=525 intron 580..710 /note="first intron" CDS 711..1000 /note="second exon (rest of L and variable region) (711 is 2nd base in codon)" /codon_start=711 misc_feature 886..886 /note="frameshift mutation (insertion of a G)" misc_feature 1003..1009 /note="heptanucleotide box, not canonical" BASE COUNT 335 a 299 c 270 g 338 t 5 others ORIGIN 1 aaatctatct atctatctat ctatctatct atctatctat ctatctatca ttcatgatta 61 taccttaang catccantgt tggtagcant gacaatttac agcactggtg tttccaggga 121 attggaccaa aaaggaagtc tctctgacct taatagtact catctgtatc aaatgcagga 181 aacttctaaa atttcttgag tttctagaga ngtttttccc tagcagacct tatcataaat 241 agaaagctag caagagaagc atgtcatgaa acatgaagag agcaaaagaa cactccacac 301 ataggacagt aggctgattc tttcctgtag cctgcaggga gaaacacang ctctgcagac 361 tttggacacc tgggaggcac tgggcctgtg cagtgttatt gagataagtc atctttgcag 421 ctgtgcagat ttgcatgtcc cacagagcaa cgcctactgc cctgaacatt tatcaatagg 481 ctggtgacat cctgtgcaga agtctctctc agtccagaca cagcatggac atgagggccc 541 ccgctcagct cctgggactc ctgctgctct ggctcccagg taaggaggga aacaacaaaa 601 attttattca gccagtgtag ccattaatgc ctggcacttc aggaaattct tcttagaaca 661 ttactaatca tgtggatatg tgtttttatg ttcctaatat cagataccag atgtgacatc 721 cagatgaccc agtctccatc ctccctgtct gcatctgtag gagacagagt caccatcact 781 tgccgggcga gtcagggcat tagcaattat ttagcctggt atcagcagaa accagggaaa 841 gttcctaagc tcctgatcta tgctgcatcc gctttgcaat cagggggtcc catctcggtt 901 cagtggcagt ggatctggga cagatttcac tctcaccatc agcagcctgc agcctgaaga 961 tgttgcaact tattactgtc aaaagtataa cagtgcccct cccactgtga tacaagcccg 1021 aacataaacc atggagggaa gtagatgtgt gagtctgggc tgccccagct gctcctcctg 1081 gtgccgccgt ctgctgacag cagttctcag atgcagccaa ggtttgaagc tccctgggaa 1141 gttttggtag aaggggtcag ggaggcacat ttacattcta tctctcttta tcctcagctc 1201 catcagctga tatgcaagta tctctcctga ttattattaa taaagga //