Path: utzoo!utgpu!watserv1!watmath!att!emory!wuarchive!uunet!stanford.edu!agate!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Human germline pseudogene for the leader peptide and variable Message-ID: Date: 27 May 91 12:26:36 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 57 Approved: lear@genbank.bio.net Checksum: 39770 3 LOCUS HUMIGK13 978 bp ds-DNA PRI 27-MAY-1991 DEFINITION Human germline pseudogene for the leader peptide and variable region of a kappa immunoglobulin (subgroup V kappa II) ACCESSION Z00012 X00904 KEYWORDS immunoglobulin; immunoglobulin light chain; kappa-immunoglobulin; pseudogene; signal peptide. SOURCE Homo sapiens DNA. ORGANISM Homo sapiens Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae. REFERENCE 1 (sites) AUTHORS Zachau,H.G. JOURNAL Unpublished (1984) STANDARD full staff_review REFERENCE 2 (bases 1 to 978) AUTHORS Pech,M., Jaenichen,H.-R., Pohlenz,H.-D., Neumaier,P.S., Klobeck,H.-G. and Zachau,H.G. TITLE Organization and evolution of a gene cluster for human immunoglobulin variable regions of the kappa type JOURNAL J. Mol. Biol. 176, 189-204 (1984) STANDARD full automatic COMMENT The sequence is designated Vc in ref. [1]; gaps are not included. From EMBL entry HSIGK13; dated 13-JUN-1985. FEATURES Location/Qualifiers intron <1..199 /note="first intron" misc_feature 200..202 /note="stop codon (pseudogene)" CDS 200..510 /note="second exon (rest of L and variable region) (200 is 1st base in codon)" /codon_start=200 misc_feature 449..449 /note="frameshift mutation (pseudogene)" misc_feature 513..519 /note="heptanucleotide box, not canonical" BASE COUNT 279 a 231 c 210 g 258 t ORIGIN 1 gatctgtgag agtgaggaag attccagaag gagcaaggat atgtaaatat tggttctttt 61 taaatctgta tgttttggga ggattaatca aaatcacaca cacaaaaata attgagcaaa 121 acataaataa cagacagaaa atgattaaaa tgactcacaa tgtttgcaca taaccttgca 181 cttctctctc attatttcat gatccaatgg agatgctgtg atgacccaac ctccactctc 241 cctgcccatc acccctggag agccagcctc catctcctgc aggtctagtc acagccccct 301 tcacagtaat ggatacatct atttcaactg gtacctacag aagacaggcc agcctccttg 361 gctcccaatc tatttggttt ccaatcacga ccctggagtc ccagacaggt tcagtggcag 421 tgggttgggg acagatttca tgctgaaatc aggaggatgg atgctgagga tgttggggtt 481 tattgctgcc agcaaagtac acattatcct cccacaatgg tatagtcttg aacacaaacc 541 cccccacttg ctgtggccta gctgcccaga tgtgctgttt ctgtggagag caggcactgt 601 ggattctctt agatgcctaa agaccaagat gttggagaac tcagaggact tggtgcagct 661 gagggctcat gaccataaat ttcttggcta cacctcaggc accacatttt aaggtcccgt 721 cagctgcagc agcctttgca tgacagagtc tgcagtatgg aggaggtcca cgtgccctct 781 gagcaatgag acacaagaga agagaaggct caatgagagc tcattctaat cctctcttcc 841 ttccctatgt tcattcatca actaaattca ttctgcataa caggcaccta attgagactg 901 attactggca acacaaaact aacacattct tttgatttgg tttagcagtt accagagtag 961 atgtagattg ataagatc //