Path: utzoo!utgpu!watserv1!watmath!att!emory!wuarchive!uunet!stanford.edu!agate!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Human germline pseudogene for the leader peptide and variable Message-ID: Date: 27 May 91 12:26:47 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 54 Approved: lear@genbank.bio.net Checksum: 51328 3 LOCUS HUMIGK7 661 bp ds-DNA PRI 27-MAY-1991 DEFINITION Human germline pseudogene for the leader peptide and variable region of a kappa immunoglobulin (subgroup V kappa I) ACCESSION Z00005 X00749 KEYWORDS germline; immunoglobulin; kappa-immunoglobulin; pseudogene; variable region. SOURCE Homo sapiens DNA. ORGANISM Homo sapiens Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae. REFERENCE 1 (bases 1 to 661) AUTHORS Jaenichen,H.R. JOURNAL Unpublished (1984) STANDARD full automatic REFERENCE 2 (bases 1 to 661) AUTHORS Jaenichen,H.R., Pech,M., Lindenmaier,W., Wildgruber,N. and Zachau,H.G. TITLE Composite human V kappa genes and a model of their evolution JOURNAL Nucleic Acids Res. 12, 5249-5263 (1984) STANDARD full automatic COMMENT The sequence is designated V55 in refs. [1] and [2]; the variable region gene segment is inactivated by several frameshift mutations From EMBL entry HSIGK7; dated 13-JUN-1985. FEATURES Location/Qualifiers CDS <1..27 /note="first exon (leader peptide) (1 is 2nd base in codon) (27 is 1st base in codon)" /codon_start=1 intron 28..151 /note="first intron" misc_feature 28..29 /note="not canonical donor splice site" CDS 152..447 /note="second exon (rest of L and variable region) (152 is 2nd base in codon)" /codon_start=152 misc_feature 450..456 /note="heptanucleotide box" BASE COUNT 162 a 167 c 152 g 180 t ORIGIN 1 cgggacttct gccatcctgg tttccagtta agaaaagaaa ttactagcct cttactcagc 61 cagtgtgcta ggtactgcct ggccattcag ggaagtcttc ttataacatg attaattgtg 121 tggatatttg tttttctgtt tgcaatttca gggaccagaa gtgacatcca gtcacccagt 181 ctccatccac actgtctgca tctgtaagga gacagcgtca ccatcacttg ccgggcgagt 241 cagagcattg gtagtaattt agactggtat cagcagaaac cagggaaggc ccctaagggc 301 ctgatctatg ctgcatccag tttgcaatct ggggctcctt cgcggttcgg tggcagtgga 361 tctgggacag attttactct caccatcaga atcctgcagc ctaaagatgt tgcaagttat 421 tactgtcaac agtataaaaa ttaccctatc acagtgttac aaaccataac aaaccccccc 481 caggaaagca gacatgtgac gctgggctgc cccacctgct cttctttgtg cagccatctg 541 gtgacaacac ttctcagact cagcctgagt tttgagggtt attgggagat tacggaagag 601 ggggccatga aggtgtctct ccaccctaag tctctttcct ctcatggcaa tgtctcttcg 661 a //