Path: utzoo!utgpu!watserv1!watmath!att!emory!sol.ctr.columbia.edu!zaphod.mps.ohio-state.edu!wuarchive!uunet!stanford.edu!agate!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Mouse gene fragment encoding dihydrofolate reductase (EC 1.5.1.3) Message-ID: Date: 27 May 91 12:37:54 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 42 Approved: lear@genbank.bio.net Checksum: 50779 3 LOCUS MUSDHF5 670 bp ds-DNA ROD 27-MAY-1991 DEFINITION Mouse gene fragment encoding dihydrofolate reductase (EC 1.5.1.3) first exon. Five fragments of this gene are given in the entries , , , and . ACCESSION V00735 KEYWORDS dihydrofolate reductase; reductase. SOURCE Mus musculus DNA. ORGANISM Mus musculus Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae. REFERENCE 1 (bases 1 to 670) AUTHORS Crouse,G.F., Simonsen,C.C., McEwan,R.N. and Schimke,R.T. TITLE Structure of amplified normal and variant dihydrofolate reductase genes in mouse sarcoma S180 cells JOURNAL J. Biol. Chem. 257, 7887-7897 (1982) STANDARD full automatic COMMENT EPD; 07057; Mm DHFR P2+. EPD; 24032; Mm DHFR P1. From EMBL entry MMDHF5; dated 13-JUL-1983. FEATURES Location/Qualifiers precursor_RNA 361..>670 /note="primary transcript" mRNA 361..528 /note="exon 1" CDS 443..528 /note="DHFR part 1 (528 is 2nd base in codon)" /codon_start=443 BASE COUNT 125 a 189 c 238 g 118 t ORIGIN 1 agacctgaag aaccggccta gaaccgtttg cctccccggg cttgggggcg gagtagagcc 61 tcccgacgcg gatttcccgg cgggcattgc agtgtgcaga agagccggcc tgctaggagc 121 gcgaggcgcg gccgcacttt ctcgcgcctg ccggcgcacg cctcaacctg tgcgggaccg 181 gccttggggg cggagcctta gctacacaaa tagaatgcgc ggcgggcctt ggtgggggcg 241 gggccttagc tgcacaaata ggatgcgcgg cgggccttgg taggggcgga gccttagctg 301 cacaaatagg atgcgcggcg ggccttggtg ggggcggggc ctaagctgcg caagtggtac 361 acagctcagg gctgcgattt cgcgccaaac ttgacggcaa tcctagcgtg aaggctggta 421 ggattttatc cccgctgcca tcatggttcg accattgaac tgcatcgtcg ccgtgtccca 481 aaatatgggg attggcaaga acggagacct accctggcct ccgctcaggt attggccggg 541 ctgggctggg aaactgaggc ggttcactga gtagggtcat gcacttggct tggacgcgcg 601 ggacgggcgt gaaagcagag cctaccggcc tcagccagag accaagctaa ccggttccac 661 gacggtgatc //