Path: utzoo!utgpu!watserv1!watmath!att!emory!wuarchive!uunet!stanford.edu!agate!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Mouse mRNA for idiotypic anti-NP IgG(1) heavy chain V-D-J Message-ID: Date: 27 May 91 12:41:32 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 56 Approved: lear@genbank.bio.net Checksum: 57147 3 LOCUS MUSIGGNP2 420 bp ss-mRNA ROD 27-MAY-1991 DEFINITION Mouse mRNA for idiotypic anti-NP IgG(1) heavy chain V-D-J (hybridoma 18.1.16) ACCESSION X02563 KEYWORDS diversity region; gamma-immunoglobulin; immunoglobulin; immunoglobulin heavy chain; joining region; signal peptide; variable region. SOURCE Mus musculus RNA. ORGANISM Mus musculus Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae. REFERENCE 1 (bases 1 to 420) AUTHORS Boersch-Supan,M.E., Agarwal,S., White-Scharf,M.E. and Imanishi-Kari,T. TITLE Heavy chain variable region Multiple gene segments encode anti-4-(hydroxy-3-nitropheny)acetyl idiotypic antibodies JOURNAL J. Exp. Med. 161, 1272-1292 (1985) STANDARD full automatic COMMENT Serological analysis of hybridomaproteins resulting from the immune response to the hapten NP reveals NP(a) idiotypes expressed by Balb/C mice and NP(b) idiotypes expressed by C57 BL/6 mice. The NP(b) family of antibodies consists of 6 subgroups (I-VI) with I-IV sharing more determinants than V and VI which appear quite distinct. From EMBL entry MMIGGNP2; dated 10-JAN-1991. FEATURES Location/Qualifiers mat_peptide join(58..351,352..375,376..420) /product="idiotypic anti-NP IgG(1) heavy chain V-D-J" CDS join(1..351,352..375,376..420) /product="idiotypic anti-NP IgG(1) heavy chain V-D-J" /codon_start=1 sig_peptide 1..57 misc_feature 58..351 misc_feature 58..351 /note="variable region (aa 1-98)" misc_feature 148..162 /note="CDR I" misc_feature 205..255 /note="CDR II" misc_feature 352..375 misc_feature 352..375 misc_feature 352..375 /note="D-region (aa 99-106)" misc_feature 376..>420 /note="J-region (aa 107-121)" BASE COUNT 107 a 106 c 110 g 97 t ORIGIN 1 atgaaatgca gctgggttat gttcttcctg atggcagtgg ttacaggggt caattcagag 61 gttcagctgc agcagtctgg ggcagagctt gtgaagccag gggcctcagt caagttgtcc 121 tgcacggctt ctggcttcaa cattaaagac acctatatgc actgggtgaa gcagaggcct 181 gaacagggcc tggagtggat tggaaggatt gatcctgcga atggtaatac taaatatgac 241 ccgaagttcc agggcaaggc cactataaca gcagacacat cctccaacac agcctacctg 301 cagctcagca gcctgacatc tgaggacact gccgtctatt actgtgctag atacctctat 361 tactacggta gtagcgactt tgactactgg ggccaaggca ccactctcac agtctcctca //